Human Recombinant PTP1B

Human Recombinant PTP1B is available 1 time from Biovision labs

  • Background
    The protein coding region of the catalytic domain of PTP-1B (amino acids 1-321) was cloned into an E. coli expression vector. The catalytic domain of PTP-1B was overexpressed in E. coli as a soluble protein, and it was purified by conventional column chromatographic techniques.
  • Concentration
    1 mg/ml
  • Molecular weight
    37.3 kDa (321 amino acids)
  • Synonym
    Human Recombinant PTP1B
  • Other name
    Protein tyrosine phosphatase, non-receptor type 1, PTPN1, Protein-tyrosine phosphatase 1B, Human Recombinant PTP1B,
  • NCBI gene number
    5770
  • NCBI gene
    PTPN1
  • Gene source
    Human
  • NCBI number
    P18031
  • Recombinant
    Yes
  • Source
    E. coli
  • Purification
    ≥95%
  • Tested applications
    SDS-PAGE, N/A
  • Level of endotoxin
    N/A
  • Tested activity
    N/A
  • Biological activity
    6,000-12,000 units/mg
  • Reesults
    N/A
  • Binding ability
    N/A
  • Unit
    Enzymatic activity is defined as the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37°C, pH 7.5 using 10 mM of substrate.
  • Storage condition
    -80°C
  • Shipping under
    gel pack
  • Physical appearance
    Liquid
  • Physical properties
    1 mg/ml solution in 25 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT, 2 mM β mercaptoethanol, 1 mM EDTA, 20% Glycerol.
  • How to reconstitute
    N/A
  • Aa sequence
    MEMEKEFEQI DKSGSWAAIY QDIRHEASDF PCRVAKLPKN KNRNRYRDVS PFDHSRIKLH QEDNDYINAS LIKMEEAQRS YILTQGPLPN TCGHFWEMVW EQKSRGVVML RVMEKGSLK CAQYWPQKEE KEMIFEDTNL KLTLISEDIK SYYTVRQLEL ENLTTQETRE ILHFHYTTWP DFGVPESPAS FLNFLFKVRE SGSLSPEHGP VVVHCSAGIG RSGTFCLADT CLLLMDKRKD PSSVDIKKVL LEMRKFRMGL IQTADQLRFS YLAVIEGAKF IMGDSSVQDQ WKELSHEDLE PPPEHIPPPP RPPKRILEPH N
  • Before use
    Centrifuge the vial prior to opening.
  • Notes
    For Research Use Only! Not to be used in humans
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
  • Short name
    Recombinant PTP1B
  • Technique
    Recombinant
  • Species
    Human
  • Alternative name
    H. sapiens recombinant PTP1B
  • Alternative technique
    rec

6301-100 | Human Recombinant PTP1Bsize: 100 μg | 454.07 USD

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