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Type
Recombinant protein
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Expression system
E.coli
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Description
Csk binding protein, also known as PAG (Phosphoprotein Associated with Glycosphingolipid-enriched microdomains) is a ubiquitously expressed transmembrane adaptor protein present in membrane rafts. Cbp/PAG has a pivotal role in the assembly of negative regulators in these membrane domains. Cbp/PAG consists of a short extracellular domain (16 to 18 residues), a transmembrane domain (20 residues), and a large cytoplasmic domain (387 to 396 residues); it contains ten tyrosine residues, nine of which are potential substrates for Src kinases, and two proline-rich regions. DNA sequence encoding intracellular domain of human Cbp/ PAG (amino acids 41- 432) was fused with a His tag at the N-terminus.
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Format
Lyophilized powder
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Purity
> 95% determined by SDS-PAGE
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Purification methd
Purified from cell culture supernatant via protein-A affinity chromatography
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Shipping and storage
Store lyophilized product in freezer (-20 °C to -80 °C). The expiration date of unopened product vial is indicated on the vial label. After reconstitution prepare suitable aliquots. Reconstituted product is stable for at least 6 months when stored in freezer (-20 °C to -80 °C). Avoid freeze/thaw cycles.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
