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Category
Primary Antibodies
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Long description
Identification of all human leukocytes, lymphocytes, monocytes, polymorphonuclear cells, eosinophils and basophils in peripheral blood expressing the 180-220 kD M. W. T200 family surface antigen. CD45 is also present on thymus, spleen, tonsil and progenitor cells in bone marrow. Human Progenitor Cell Antigen (MW 115 K daltons) is found on normal peripheral blood lymphocytes, monocytes, granulocytes and platelets. CD34 is present on 1% or less of cells in normal human bone marrow. Human Progenitor Cell Antigen reacts with 30-70% of acute leukemias, as well as all TdT positive lymphocytes in bone marrow.
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Antibody come from
CD45=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells of BALB/c mice immunized with peripheral blood monocytes from rheumatoid arthritis patient CD34=Derived from the hybridization of mouse Sp2/O-Ag14 cells with spleen cells of BALB/c mice immunized with human cell line KG-1a
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Other description
Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein. Protein A/G Chromatography
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Clone
not specified
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Antigen antibody binding interaction
Mouse anti CD45 antibody conjugated to FITC - CD34 antibody conjugated to PE Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
see techfile
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Research interest
CD Marker
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Application
Flow Cytometry
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Antibody s suited for
PBMC: Add10µl of MAB/10^6 PBMC in 100µl PBS. Mix gently and incubate for 15 minutes at 2¼ to 8¼C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20¼C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer s insBlood and Immunofluorescence analysis with a flow cytometer or microscope.
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Storage
4ºC
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Relevant references
1 .Leukocyte TypingIV: White Cell Differentiation Antigens, Schwinzer R. Cluster report: CD45/CD45R, Knapp W., Dorken B., Gilks W.R., et al, eds. Oxford University Press; 1989:628-634 _x000B__x000B_2.Association of CD2 and CD45 on human T lymphocytes. Schraven B., Samstag Y., Altevogt P., Meuer S.C., Nature 1990 May ;345(6270):71-4 _x000B__x000B_3. Antigenic Analysis of Hematopoiesis III. A Hematopoietic Progenitor Cell Antigen Defined by a Monoclonal Antibody raised against KG-1a cells Civin, C.I., Strauss,L.C., Broveal,C., Fackler,M.J., Schwartz,J.F., and Shaper,J.H., J. Immunol. 133,157,1984 _x000B__x000B_4. Rapid isolation of human CD34 hematopoietic stem cells--purging of human tumor cells. Lebkowski JS; Schain LR; Okrongly D; Levinsky R; Harvey MJ; Okarma T Transplantation 1992 May;53(5):1011-9 _x000B__x000B_5. Distribution of surface-membrane molecules on bone marrow and cord blood CD34+ hematopoietic cells. Saeland S; Duvert V; Caux C; Pandrau D; Favre C; Valle A; Durand I; Charbord P; de Vries J; Banchereau J Exp Hematol 1992 Jan;20(1):24-33 _x000B__x000B_6. CD34-positive cell proportions in peripheral blood correlate with colony-forming capacity. Fritsch G; Emminger W; Buchinger P; Printz D; Gadner H Exp Hematol 1991 Dec;19(11):1079-83 _x000B__x000B_7. CD34 expression by stromal precursors in normal human adult bone marrow. Simmons PJ; Torok-Storb B Blood 1991 Dec 1;78(11):2848-53 _x000B__x000B_8. Human CD34+ HLA-DR- bone marrow cells contain progenitor cells capable of self-renewal, multilineage differentiation, and longterm in vitro hematopoiesis. Srour EF; Brandt JE; Briddell RA; Leemhuis T; van Besien K; Hoffman R Blood Cells 1991;17(2):287-95 _x000B__x000B_9. Surface marker expression in acute myeloid leukaemia at first relapse. Thomas X; Campos L; Archimbaud E; Shi ZH; Treille-Ritouet D; Anglaret B; Fiere D Br J Haematol 1992 May;81(1):40-4 _x000B__x000B_10. Long-term erythropoiesis from constant numbers of CD34+ cells in serum-free cultures initiated with highly purified progenitor cells from human bone marrow. Lansdorp PM; Dragowska W J Exp Med 1992 Jun 1;175(6):1501-9
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Stem cell or hematopoietic marker. The most used affinity clone is QBEND10. We compare the affinity to this clone. This nordc Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. nordc FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
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Gene target
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Gene symbol
CD34, PTPRCAP, PTPRC
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Short name
Mouse anti CD45 antibody conjugated FITC - CD34 antibody conjugated PE
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Technique
Antibody, Mouse, anti, FITC, antibody to, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, mouses
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Host
mouse
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Isotype
IgG1 (F)/IgG1 (PE)
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Label
FITC and PE
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Species
Mouse, Mouses
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Alternative name
Mouse antibody to CD45 (antibody to-) coupled to fluorecein - CD34 molecule (antibody to-) coupled to peroxidase
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Alternative technique
antibodies, murine, fluorescine
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Alternative to gene target
CD34 molecule, CD34 and IDBG-106399 and ENSG00000174059 and 947, sulfate binding, Extracellular, Cd34 and IDBG-209317 and ENSMUSG00000016494 and 12490, CD34 and IDBG-638449 and ENSBTAG00000010562 and 281051
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