-
Category
Primary Antibodies
-
Long description
Identification of all human leukocytes, lymphocytes, monocytes, polymorphonuclear cells, eosinophils and basophils in peripheral blood expressing the 180-220 kDa M.W. T200 family surface antigen. CD45 is also present on thymus, spleen, tonsil and progenitor cells in bone marrow. CD14 is present on 70-93% of Monocyte/Macrophage cells in normal peripheral blood. Identification of human Monocyte/Macrophage cells expressing the 55 kDa M.W. surface antigen
-
Antibody come from
CD45=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells of BALB/c mice immunized with peripheral blood monocytes from rheumatoid arthritis patient
-
Other description
Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein. Protein A/G Chromatography
-
Clone
not specified
-
Antigen antibody binding interaction
Mouse anti CD45 antibody conjugated to FITC - CD14 antibody conjugated to PE Antibody
-
Antibody is raised in
Mouse
-
Antibody s reacts with
Human
-
Antibody s reacts with these species
This antibody doesn't cross react with other species
-
Antibody s specificity
No Data Available
-
Research interest
CD Marker
-
Application
Flow Cytometry
-
Antibody s suited for
PBMC: Add10µl of MAB/10^6 PBMC in 100µl PBS. Mix gently and incubate for 15 minutes at 2¼ to 8¼C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20¼C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturerÕs instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
-
Storage
4ºC
-
Relevant references
1 .Leukocyte TypingIV: White Cell Differentiation Antigens, Schwinzer R. Cluster report: CD45/CD45R, Knapp W., Dorken B., Gilks W.R., et al, eds. Oxford University Press; 1989:628-634 _x000B__x000B_2.Association of CD2 and CD45 on human T lymphocytes. Schraven B., Samstag Y., Altevogt P., Meuer S.C., Nature 1990 May ;345(6270):71-4 _x000B__x000B_3. Immunocytochemical investigation of normal and chronic lymphocytic leukaemia lymphocytes reveals unexpectedly frequent reactivity with some myelomonocytic associated antibodies. Woessner S; Florensa L; Besses C; Vila RM; Galles C; Palou L; Sabrafen JS Leuk Res 1992;16(5):505-10 _x000B__x000B_4. Effect of cytokines and lipopolysaccharide on CD14 antigen expression in human monocytes and macrophages. Landmann R; Ludwig C; Obrist R; Obrecht JP J Cell Biochem 1991 Dec;47(4):317-29 _x000B__x000B_5. Surface markers in adult acute myeloblastic leukemia: correlation of CD19+, CD34+ and CD14+/DR--phenotypes with shorter survival. Groupe d'Etude Immunologique des Leucemies (GEIL). Solary E; Casasnovas RO; Campos L; Bene MC; Faure G; Maingon P; Falkenrodt A. Leukemia 1992 May;6(5):393-9 _x000B__x000B_6. In situ hybridization of interleukin-1 in CD14-positive cells in rheumatoid arthritis. Wood NC; Dickens E; Symons JA; Duff GW Clin Immunol Immunopathol 1992 Mar;62(3):295-300 _x000B__x000B_7. Expression of CD54, CD58, CD14, and HLA-DR on macrophages and macrophage-derived accessory cells and their accessory capacity. Ocklind G; Friedrichs D; Peters JH Immunol Lett 1992 Feb 15;31(3):253-8 _x000B__x000B_8. IL-4 decreases the expression of the monocyte differentiation marker CD14, paralleled by an increasing accessory potency. Ruppert J; Friedrichs D; Xu H; Peters JH Immunobiology 1991 Aug;182(5):449-64
-
Protein number
see ncbi
-
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This nordc Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. nordc FITC is highly sensitive to pH extremes.
-
Conjugation
Anti-FITC Antibody
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus
-
French translation
anticorps
-
Gene target
-
Gene symbol
CD14, PTPRCAP, PTPRC
-
Short name
Mouse anti CD45 antibody conjugated FITC - CD14 antibody conjugated PE
-
Technique
Antibody, Mouse, anti, FITC, antibody to, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, mouses
-
Host
mouse
-
Isotype
IgG1 (F)/IgG1 (PE)
-
Label
FITC and PE
-
Species
Mouse, Mouses
-
Alternative name
Mouse antibody to CD45 (antibody to-) coupled to fluorecein - CD14 molecule (antibody to-) coupled to peroxidase
-
Alternative technique
antibodies, murine, fluorescine
-
Alternative to gene target
CD14 molecule, CD14 and IDBG-49157 and ENSG00000170458 and 929, lipoteichoic acid binding, Extracellular, Cd14 and IDBG-138525 and ENSMUSG00000051439 and 12475, CD14 and IDBG-646064 and ENSBTAG00000015032 and 281048
-
