CD34 Antibody, FITC Conjugated
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Catalog number
bs-2038R-FITC
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Price
Please ask
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Size
0.1ml
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Long name
CD34 Polyclonal Antibody, FITC Conjugated
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Category
Conjugated Primary Antibodies
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Conjugation
FITC
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Host Organism
Rabbit (Oryctolagus cuniculus)
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Target Antigen
CD34
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Specificity
This is a highly specific antibody against CD34.
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Modification
Unmodified
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Modification site
None
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Clonality
Polyclonal
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Clone
Polyclonal antibody
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Concentration
1ug per 1ul
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Immunogen_range
240-280/382
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Subcellular location
Extracellular
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Source
KLH conjugated synthetic peptide derived from mouse CD34
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Gene ID number
12490
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Swiss Prot
Q64314
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Tested applications
FCM, IF(IHC-P), IF(ICC)
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Recommended dilutions
FCM(1:20-100), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)
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Crossreactivity
Human, Mouse, Rat, Rabbit
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Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Antigen background
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins (By similarity).
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Purification method
This antibody was purified via Protein A.
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Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
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Excitation Emission
494nm/518nm
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Synonyms
AU496; Hematopoietic progenitor cell antigen CD34; CD34
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Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Stem cell or hematopoietic marker. The most used affinity clone is QBEND10. We compare the affinity to this clone. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
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Additional conjugation
Anti-FITC Antibody
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French translation
anticorps
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Gene target
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Gene symbol
CD34
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Short name
Anti-CD34
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Technique
Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
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Isotype
Immunoglobulin G (IgG)
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Label
FITC
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Alternative name
Anti-CD34 PAb FITC
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Alternative technique
antibodies, fluorescine
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Alternative to gene target
CD34 molecule, CD34 and IDBG-106399 and ENSG00000174059 and 947, sulfate binding, Extracellular, Cd34 and IDBG-209317 and ENSMUSG00000016494 and 12490, CD34 and IDBG-638449 and ENSBTAG00000010562 and 281051
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Gene info
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Identity
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Gene
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Long gene name
CD34 molecule
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Synonyms gene name
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GenBank acession
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Locus
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Discovery year
1990-09-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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