Recombinant human MSLN(296-580) protein with C-terminal mouse Fc and 6xHis tag
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Catalog number
MBS188087
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Price
Please ask
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Size
INQUIRE
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Products_type
Recombinant Protein
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Products_short_name
[MSLN]
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Products_name_syn
[Human MSLN(296-580) Protein, mFc-His Tag; MSLN; Mesothelin; MPF]
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Other_names
[mesothelin isoform 1 preproprotein; Mesothelin; mesothelin; CAK1 antigen; megakaryocyte potentiating factor; soluble MPF mesothelin related protein; pre-pro-megakaryocyte-potentiating factor; mesothelin; CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor]
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Products_gene_name
[MSLN]
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Other_gene_names
[MSLN; MSLN; MPF; SMRP; MPF; MPF]
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Purity
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
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Form
Lyophilized from sterile PBS, pH 7.4. Normally 5%-38% trehalose is added as protectants before lyophilization.
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Storage_stability
Store at -80 degree C for 12 months (Avoid repeated freezing and thawing)Shipping: Blue Ice
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Tested_application
Western Blot (WB)
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Conjugated
The polyhistidine (6xHis) tag or Histidine Epitope Tag (HIS Tag, 6XHIS Tag with Histidine (abbreviated as His or H; encoded by the codons CAU and CAC) is an α-amino acid that is used in the biosynthesis of proteins. It is the most used tag for purification by Affinity Chromatography (IMAC). The pentadentate chelator is the best chelator in Immobilized Metal ion Affinity chromatography (IMAC) for the6xhis-tagged proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
MSLN, MIR296, MIR580
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Short name
Recombinant MSLN(296-580) protein with C-terminal mouse Fc tag
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Technique
Recombinant, Mouse, FC, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. MyBioSource advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Host
HEK293 cells
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Label
tag
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Species
Mouse, Humans, Mouses
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Alternative name
Rec. H. sapiens mesothelin(296-580) protein including C-terminal mouse fragment c and 6xHis detection labelled
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Alternative technique
rec, murine
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Alternative to gene target
mesothelin, MPF and SMRP, MSLN and IDBG-8270 and ENSG00000102854 and 10232, protein binding, Extracellular, Msln and IDBG-152832 and ENSMUSG00000063011 and 56047, MSLN and IDBG-634054 and ENSBTAG00000000177 and 516237
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Gene info
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Identity
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Gene
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Long gene name
mesothelin
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Synonyms
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GenBank acession
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Locus
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Discovery year
1999-11-22
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Entrez gene record
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Pubmed identfication
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
microRNA 296
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
Gene info
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Identity
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Gene
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Long gene name
microRNA 580
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2006-05-16
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Entrez gene record
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RefSeq identity
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Classification
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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