Recombinant human TNFalpha protein with C-terminal 6xHis tag
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Catalog numberMBS188118
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Price:
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SizeINQUIRE
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Products_typeRecombinant Protein
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Products_short_name[TNFalpha]
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Products_name_syn[Human TNFalpha Protein, His Tag; DIF;TNF-alpha;TNFA;TNFSF2;cachexin;cachectin;TNFalpha]
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Other_names[tumor necrosis factor; Tumor necrosis factor; tumor necrosis factor; TNF-a; cachectin; APC1 protein; TNF, monocyte-derived; TNF, macrophage-derived; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-aCleaved into the following 6 chains:Tumor necrosis factor, membrane form; Alternative name(s):; N-terminal fragment]
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Products_gene_name[TNFalpha]
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Other_gene_names[TNF; TNF; DIF; TNFA; TNFSF2; TNF-alpha; TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2]
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PurityThe purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
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FormLyophilized from sterile PBS, pH 7.4. Normally 5%-67% trehalose is added as protectants before lyophilization.
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Storage_stabilityStore at -80 degree C for 12 months (Avoid repeated freezing and thawing)Shipping: Blue Ice
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Tested_applicationWestern Blot (WB)
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ConjugatedThe polyhistidine (6xHis) tag or Histidine Epitope Tag (HIS Tag, 6XHIS Tag with Histidine (abbreviated as His or H; encoded by the codons CAU and CAC) is an α-amino acid that is used in the biosynthesis of proteins. It is the most used tag for purification by Affinity Chromatography (IMAC). The pentadentate chelator is the best chelator in Immobilized Metal ion Affinity chromatography (IMAC) for the6xhis-tagged proteins.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Short nameRecombinant TNFalpha protein with C-terminal tag
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. MyBioSource advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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HostHEK293 cells
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Labeltag
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SpeciesHuman, Humans
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Alternative nameRec. H. sapiens TNFalpha protein including C-terminal 6xHis detection labelled
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Alternative techniquerec
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MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data