Recombinant human SIGLEC15 protein with C-terminal mouse Fc and 6xHis tag

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• Catalog number
  MBS188139
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# #

• Products_type
  Recombinant Protein
• Products_short_name
  [SIGLEC15]
• Products_name_syn
  [Human SIGLEC15 Protein, mFc-His Tag; CD33 antigen-like 3;SIGLEC-15;CD33L3;sialic acid-binding Ig-like lectin 15;Siglec15;Siglec-15]
• Other_names
  [sialic acid-binding Ig-like lectin 15; Sialic acid-binding Ig-like lectin 15; sialic acid-binding Ig-like lectin 15; CD33 antigen-like 3; CD33 molecule-like 3; sialic acid binding Ig-like lectin 15; CD33 antigen-like 3]
• Products_gene_name
  [SIGLEC15]
• Other_gene_names
  [SIGLEC15; SIGLEC15; CD33L3; HsT1361; SIGLEC-15; CD33L3; Siglec-15]
• Purity
  The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• Form
  Lyophilized from sterile PBS, pH 7.4. Normally 5%-79% trehalose is added as protectants before lyophilization.
• Storage_stability
  Store at -80 degree C for 12 months (Avoid repeated freezing and thawing)Shipping: Blue Ice
• Tested_application
  Western Blot (WB)
• Conjugated
  The polyhistidine (6xHis) tag or Histidine Epitope Tag (HIS Tag, 6XHIS Tag with Histidine (abbreviated as His or H; encoded by the codons CAU and CAC) is an α-amino acid that is used in the biosynthesis of proteins. It is the most used tag for purification by Affinity Chromatography (IMAC). The pentadentate chelator is the best chelator in Immobilized Metal ion Affinity chromatography (IMAC) for the6xhis-tagged proteins.
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus
• Source
  Recombinants or rec. proteins
• Group
  recombinants

#

• Gene target
  SIGLEC15   protein   with   tag  
• Gene symbol
  SIGLEC15
• Short name
  Recombinant SIGLEC15 protein with C-terminal mouse Fc tag
• Technique
  Recombinant, Mouse, FC, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. MyBioSource advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Host
  HEK293 cells
• Label
  tag
• Species
  Mouse, Humans, Mouses
• Alternative name
  Rec. H. sapiens SIGLEC15 protein including C-terminal mouse fragment c and 6xHis detection labelled
• Alternative technique
  rec, murine

Gene info

• Identity
  HGNC:27596
• Gene
  SIGLEC15
• Long gene name
  sialic acid binding Ig like lectin 15
• Synonyms gene
  • CD33L3
• Synonyms gene name
  • CD33 antigen-like 3
  • CD33 molecule-like 3
  • sialic acid binding Ig-like lectin 15
• Synonyms
  • HsT1361
• GenBank acession
  • AK095432
• Locus
  18q21.1
• Discovery year
  2004-01-16
• Entrez gene record
  284266
• Pubmed identfication
  • 17483134
• RefSeq identity
  • NM_213602
• Classification
  • Sialic acid binding Ig like lectins
  • V-set domain containing
• VEGA ID
  OTTHUMG00000170870

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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