Recombinant Human ACE2 His and FLAG Tag

• Catalog number
  MBS669487
• Price
  Please ask
• Size
  0.05 mg

• Products_type
  Recombinant Protein
• Products_short_name
  [ACE2]
• Products_gene_name
  [ACE2]
• Purity
  >95% by SDS-PAGE
• Form
  0.5mg/ml in sterile PBS with 20% Glycerol, pH 7.4
• Storage_stability
  It is shipped on ice packs. Upon arrival, store at -20 degree C. Do not freeze-thaw multiple times.
• Tested_application
  Functional Assay (FA), ELISA (EIA)
• Properties
  An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Conjugation
  Flag, histidine
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  ACE2   His   Tag  
• Gene symbol
  ACE2
• Short name
  Recombinant ACE2 His Tag
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. MyBioSource advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Host
  CHO cells
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 histidine and FLAG detection labelled
• Alternative technique
  rec
• Alternative to gene target
  angiotensin I converting enzyme (peptidyl-dipeptidase A) 2, ACE2 and IDBG-46534 and ENSG00000130234 and 59272, peptide binding, Extracellular, Ace2 and IDBG-184519 and ENSMUSG00000015405 and 70008, ACE2 and IDBG-638902 and ENSBTAG00000034402 and 101908244,509235

Gene info

• Identity
  HGNC:13557
• Gene
  ACE2
• Long gene name
  angiotensin converting enzyme 2
• Synonyms gene name
  • angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
  • angiotensin I converting enzyme 2
• Synonyms
  • ACEH
• Synonyms name
  • peptidyl-dipeptidase A
• GenBank acession
  • AF291820
• Locus
  Xp22.2
• Discovery year
  2000-09-25
• Entrez gene record
  59272
• Pubmed identfication
  • 10969042
• VEGA ID
  OTTHUMG00000021177
• Locus Specific Databases
  • Global Variome shared LOVD

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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