FITC antibody
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Catalog numberMBS531027
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PricePlease ask
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Size1 mg
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Products_typeAntibody
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Products_short_name[FITC]
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Products_name_syn[Monoclonal FITC antibody; Anti-FITC antibody; Fluorescein antibody]
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Other_names[FitC; FitC; FitC]
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Products_gene_name[FITC]
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Other_gene_names[fitC]
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ClonalityMonoclonal
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Clone[M25286]
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ReactivityFITC 100%
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FormChromatography purified IgG fraction, supplied in 15mM PBS, pH 7.2, with 0.1% sodium azide.
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Concentration3.66mg/ml by OD280nm
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Storage_stabilityShort term: 4°C; Long term: -20°C
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Tested_applicationUser optimized
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PropertiesIf you buy Antibodies supplied by MyBioSource they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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French translationanticorps
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Short nameFITC antibody
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TechniqueAntibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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HostMouse
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IsotypeIgG2a, lambda
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LabelFITC
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Alternative namefluorecein (antibody to-)
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Alternative techniqueantibodies, fluorescine
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MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data