FITC antibody
-
Catalog number10R-F121a
-
PricePlease ask
-
Size1 mg
-
-
CategoryPrimary Antibody
-
Antibody SubtypeMonoclonal Antibodies
-
Area of researchMiscellaneous
-
Type of ImmunogenFITC antibodies were raised in mouse using FITC-BSA conjugate ..
-
Raised inMouse
-
Cross ReactivityShould be determined by the end user
-
IsotypesIgG2a
-
Clone2A3
-
Method of PurificationFITC antibody was purified by chromatography on protein A Sepharose.
-
Form BufferProtein A sepharose purified and Provided in PBS, pH 7.4, 0.1 % NaN3.
-
StorageStore at 4 deg C.
-
Shipping conditionsBlue Ice
-
Tested forIF; IHC
-
Usage RecommendationsOptimal conditions to be determined by end user
-
PropertiesIf you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
-
ConjugationAnti-FITC Antibody
-
French translationanticorps
-
Short nameFITC antibody
-
TechniqueAntibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
-
LabelFITC
-
Alternative nameMouse monoclonal FITC antibody
-
Alternative techniqueantibodies, fluorescine
-
MeSH Data
-
Name
-
ConceptScope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
-
Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data