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Long name
FITC Goat Anti-Mouse IgG (H+L)
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Antigen
FITC
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Synonim names
N/A
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Other names
FitC; FitC; FitC
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Gene name
FITC ; FITC
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Gene synonims
fitC; N/A
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Category
Antibodies
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Clonality
N/A
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Clone
Not applicable to Polyclonal Antibodies
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Reacts with
Due to limitted amount of tested species we cannot guarantee that no crossreactivity will occur.
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Specificity
This is an antibody designed to detect FITC ; FITC
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Purification method
The most common purification methods used in the antibody production are Affinity Chromatography, Caprylic Acid Ammonium Sulfate Precipitation, Antigen Affinity method, etc.
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Form Appearance
N/A
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Concentration
Enquire us for detailed information on the concentration of this antibody
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Storage shipping and handling
The antibody is shipped at +4 degrees Celsius. Upon receving, freeze at -20. For longer periods of time we recommend keeping the vial frozen at -40 or -80. Avoid cycles of freezing and thawing as they may denaturate the polypeptide chains of the antibody. Due to transportation or handling reasons, small amounts of the antibody might get caught on the lid or walls of the vial. We recommend you to briefly cetrifuge the vial prior to use to gather the content on the bottom.
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Applications
Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
If you buy Antibodies supplied by MBS Polyclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Polyclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Polyclonals FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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French translation
anticorps
