FITC antibody

• Catalog number
  70R-FG001
• Price
  Please ask
• Size
  1 mg

• Category
  Primary Antibody
• Antibody Subtype
  Polyclonal Antibodies, Purified
• Area of research
  Miscellaneous
• Type of Immunogen
  FITC antibodies were raised in goat using fluorescein ..
• Raised in
  Goat
• Cross Reactivity
  Should be determined by the end user
• Isotypes
  IgG
• Method of Purification
  FITC antibody was purified by affinity chromatography.
• Concentration
  1.0 mg/ml
• Form Buffer
  Provided as a liquid in PBS, pH 7.2, with 0.1% NaN3.
• Storage
  Store at 4 deg C.
• Shipping conditions
  Blue Ice
• Tested for
  ELISA; ICC; IHC; WB
• Usage Recommendations
  ELISA: coating:1:100-1:500, primary:1:1,000-1:30,000, ICC: 1:200-1:2,000,IHC: 1:200-1:2,000, WB: colorimetric: 1:1,000-1:10,000, chemiluminescent: 1:1,000-1:30,000
• URL
• More info
• Properties
  If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Short name
  FITC antibody
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
• Label
  FITC
• Alternative name
  Affinity purified Goat polyclonal FITC antibody
• Alternative technique
  antibodies, fluorescine

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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