Mouse IL-12/-23 (p40) ELISpotBASIC kit (HRP)

Categories

ELISpot Kits
Description

The monoclonal antibodies in this kit are specific to the p40 subunit of IL-12 and IL-23. ELISpot assays based on antibodies to p40 will detect both IL-12 and IL-23. Such assays can also detect p40 monomers and dimers.
Enzyme Conjugate

HRP
Format

without plates
Kit Detection Type

Streptavidin-HRP
Application

ELISpot
Reactivities

mouse
Analytes

IL12/23 (p40)
Contents

Capture mAb (C15.6),Biotinylated detection mAb (C17.8),Streptavidin-HRP
Datasheet
Product Header

Basic ELISpot kit for enumeration of cells secreting IL-12/-23 (p40)
GeneID

NA
Shelf Life

18 months
Properties

HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
Conjugation

HRP Conjugated
Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name

Mus musculus

Gene target

IL-12 p40 ELISpotBASIC kit
Gene symbol

IFNLR1, PIRC55, PIRC53, PIRC54, PIRC57, PIRC56, SNORD116-12, IGHV3OR16-12, SNORD114-12
Short name

Mouse IL-12/-23 (p40) ELISpotBASIC kit ( )
Technique

Mouse, mouses
Host

mouse
Label

HRP
Species

Mouse, Mouses
Alternative name

Mouse Interleukin-12/-23 (p40) ELISpotBASIC reagent (horseradish peroxidase)
Alternative technique

murine, kits
Alternative to gene target

v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Identity

HGNC:18584
Gene

IFNLR1
Long gene name

interferon lambda receptor 1
Synonyms gene
- IL28RA
Synonyms gene name
- interleukin 28 receptor, alpha
- interleukin 28 receptor, alpha (interferon, lambda receptor)
- interferon, lambda receptor 1
Synonyms
- CRF2/12
- IFNLR
- IL-28R1
GenBank acession
- AY129153
Locus

1p36.11
Discovery year

2002-12-02
Entrez gene record

163702
RefSeq identity
- NM_170743
Classification
- Interferon receptors
VEGA ID

OTTHUMG00000003036

Name

Enzyme-Linked Immunospot Assay
Concept

Scope note: A method of detection of the number of cells in a sample secreting a specific molecule. With this method, a population of cells are plated over top of the immunosorbent substrate that captures the secreted molecules.
Tree numbers
- E01.370.225.500.508
- E05.200.500.508
- E05.242.551
- E05.478.566.350.170.500
- E05.478.566.380.360.500
- E05.478.583.400.170.500
- E05.601.470.350.170.500
- E05.601.470.380.360.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data