Mouse IgG2c ELISpotBASIC kit (HRP)
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Catalog number3824-2H
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Price:
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Sizefor 4 plates
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CategoriesELISpot Kits
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DescriptionWith this kit, detection of antigen-specific IgG2c secreting B cells can be performed using coated antigen. If detection with biotinylated antigen is preferred, the plate should instead be coated with the subclass specific antibody, which is available in the web shop.
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Enzyme ConjugateNA
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Formatwithout plates
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Kit Detection TypeStreptavidin-HRP
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ApplicationELISpot
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Reactivitiesmouse
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AnalytesIgG2c
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ContentsCapture mAbs (MT24/JC5-1),Biotinylated detection mAb (MTG2c),Streptavidin-HRP,R848,Recombinant mouse IL-2
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Product HeaderBasic ELISpot for enumeration of IgG2c secreting B cells
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GeneIDNA
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Shelf Life18 months
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PropertiesHRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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ConjugationHRP Conjugated
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Short nameMouse IgG2c ELISpotBASIC kit ( )
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TechniqueMouse, mouses
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Hostmouse
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IsotypeIgG2c
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LabelHRP
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SpeciesMouse, Mouses
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Alternative nameMouse IgG2c ELISpotBASIC reagent (horseradish peroxidase)
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Alternative techniquemurine, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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MeSH Data
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Name
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ConceptScope note: A method of detection of the number of cells in a sample secreting a specific molecule. With this method, a population of cells are plated over top of the immunosorbent substrate that captures the secreted molecules.
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Tree numbers
- E01.370.225.500.508
- E05.200.500.508
- E05.242.551
- E05.478.566.350.170.500
- E05.478.566.380.360.500
- E05.478.583.400.170.500
- E05.601.470.350.170.500
- E05.601.470.380.360.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data