Blasticidin solution, 10mg/ml in 20mM HEPES
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Catalog numberB006-20 ml
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Price:
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Size20ml
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Chemical formulaC17H26N8O5*HCl
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CAS number3513-03-9
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Percentage purity or assay gradeRequest
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Ordering informationIf you wish to order this item, please send us by email or by fax an official purchase order including your full shipping and billing information. Contact us if you need first a quotation. Delivery for USA and Europe next week if an order is received before Friday 2 pm.
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Shipping conditiondry ice
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Storage condition-20°C
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Buffer25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
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DescriptionThe volume (ml) in milliliters of buffered % w/v solutions at the medium pH are also used for DNA extraction Organic (Phenol-Chloroform) Extraction, Non-Organic (Proteinase K and Salting out), Chelex (Ion Exchange Resin) Extraction, EDTA or PBS aqueous.
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Gene target
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Short nameBlasticidin , 10mg/ in 20mM
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Alternative nameBlasticidin solution, 10mg/milliliter in 20mM HEPES
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