HBSS with 20mM HEPES

How to order

The reference at Genprice for this lab product is 271-20011. It is a frequent ordered reagent.
When will I be delivered

The HBSS with 20mM HEPES will be delivered to your laboratory the next week after your order if you order it before Friday 15:30.
Contact with other users

HBSS with 20mM HEPES is frequently used in research laboratories. We can supply you with references if needed.
Buffer

25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.