Nutrient Mixture F-10 Ham w/ 20mM HEPES buffer w/o L-Glutamine and Sodium bicarbonate

  • Catalog number
    AL196-30X500ML
  • Price
    Please ask
  • Size
    30X500ML
  • Description
    Nutrient Mixture F-10 Ham, w/ 20mM HEPES buffer w/o L-Glutamine and Sodium bicarbonate, is an orangish red colored, clear solution. This mixture was developed for single cell plating of near diploid Chinese hamster ovary (CHO) cells and mouse L-cells. It is now widely used for culturing a variety of cells which include human diploid cells and white blood cells for chromosomal analysis and primary explants of rat, rabbit and chicken tissues.
  • Similar products
  • Shelf life
    Use before expiry date given on the product label.
  • Storage
    Store at 2-8°C away from bright light
  • Appearance
    Orangish red colored, clear solution
  • PH
    NA
  • Osmolality in mOsm Kg H2O
    NA
  • Sterility
    NA
  • Cultural Response
    NA
  • Endotoxin Content
    NA
  • Uses
    Laboratory chemicals, Manufacture of substances
  • Personal Protection
    Use personnel protective equipment. Wear disposable gloves, dust mask and eye protection. Avoid dust formation.
  • Toxicological Information
    NA
  • Technical Data
    Contact us to request a Technical Data
  • Data sheet
  • Ordering
    To order Nutrient Mixture F-10 Ham w/ 20mM HEPES buffer w/o L-Glutamine and Sodium bicarbonate, please use the Cat. Nr.AL196-30X500ML and submit your purchase order by email or by fax. A discount is available for larger or bulk quantities, please contact us for more information
  • Tips
    Our specialists recommend you to follow carefully the pre-registered instructions for Nutrient Mixture F-10 Ham w/ 20mM HEPES buffer w/o L-Glutamine and Sodium bicarbonate
  • Additional description
    Buffering solutions are useful to keep the pH range sable when using this reagent of Himedia.
  • Buffer
    25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.
  • Gene target
    Nutrient   Mixture   F-10   Ham   20mM   w/o   L-Glutamine   Sodium   bicarbonate  
  • Gene symbol
    F10, F10-AS1, HLA-F, H3C11, IGHV1-69-2, SNAR-F, PIRC49, KLRC4, SNORD115-10
  • Short name
    Nutrient Mixture F-10 Ham w/ 20mM w/o L-Glutamine Sodium bicarbonate
  • Alternative name
    Nutrient Mixture F-10 Ham w/ 20mM HEPES solution w/o L-Glutamine and Sodium bicarbonate
Gene info
Gene info
  • Identity
    HGNC:40225
  • Gene
    F10-AS1
  • Long gene name
    F10 antisense RNA 1
  • Synonyms gene name
    • F10 antisense RNA 1 (non-protein coding)
  • Locus
    13q34
  • Discovery year
    2011-05-09
  • Classification
    • Antisense RNAs
  • VEGA ID
    OTTHUMG00000133691
Gene info
Gene info
Gene info
Gene info
  • Identity
    HGNC:34326
  • Gene
    SNAR-F
  • Long gene name
    small NF90 (ILF3) associated RNA F
  • Synonyms gene name
    • small nuclear ILF3/NF90-associated RNA F
    • small ILF3/NF90-associated RNA F
  • GenBank acession
  • Locus
    19q13.33
  • Discovery year
    2008-06-20
  • Entrez gene record
    100126781
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Small NF90 (ILF3) associated RNAs
Gene info
  • Identity
    HGNC:37553
  • Gene
    PIRC49
  • Long gene name
    piwi-interacting RNA cluster 49
  • Locus
    10
  • Discovery year
    2009-11-05
  • Entrez gene record
    100313929
  • Pubmed identfication
  • Classification
    • Piwi-interacting RNA clusters
Gene info
  • Identity
    HGNC:6377
  • Gene
    KLRC4
  • Long gene name
    killer cell lectin like receptor C4
  • Synonyms gene name
    • killer cell lectin-like receptor subfamily C, member 4
  • Synonyms
  • GenBank acession
  • Locus
    12p13.2
  • Discovery year
    1998-09-03
  • Entrez gene record
    8302
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Killer cell lectin like receptors
  • VEGA ID
    OTTHUMG00000168575
Gene info
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