5 x HEPES Buffer B Solution

• Catalog number
  BH016
• Price
  Please ask
• Size
  2X100ml

• St
  R
• Description
  Buffering solutions are useful to keep the pH range sable when using this reagent of iNtRON. The volume (ml) in milliliters of buffered % w/v solutions at the medium pH are also used for DNA extraction Organic (Phenol-Chloroform) Extraction, Non-Organic (Proteinase K and Salting out), Chelex (Ion Exchange Resin) Extraction, EDTA or PBS aqueous.
• Buffer
  25mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) pH7 solution buffers are a media with a zwitterion organic chemical buffering agent; one of the twenty Good's buffers. HEPES DMEM medium is widely used in cell culture, largely because it is better at maintaining physiological pH 7 tot 7,5 despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES, 25 mM in RPMI and DMEM is like water in that its dissociation decreases as the temperature decreases. This makes HEPES RPMI 25 milli-molar media with or without L-glutamine a more effective buffering agent for maintaining enzyme structure and function at low temperatures.

• Gene target
  -
• Gene symbol
  CXCL5, LAMTOR5
• Short name
  5 x B
• Alternative name
  5 x HEPES solution B solution

Gene info

• Identity
  HGNC:10642
• Gene
  CXCL5
• Long gene name
  C-X-C motif chemokine ligand 5
• Synonyms gene
  • SCYB5
• Synonyms gene name
  • small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78)
  • chemokine (C-X-C motif) ligand 5
• Synonyms
  • ENA-78
• GenBank acession
  • X78686
• Locus
  4q13.3
• Discovery year
  1996-09-03
• Entrez gene record
  6374
• Pubmed identfication
  • 7929219
• RefSeq identity
  • NM_002994
• Classification
  • Chemokine ligands
• VEGA ID
  OTTHUMG00000160871

Gene info

• Identity
  HGNC:17955
• Gene
  LAMTOR5
• Long gene name
  late endosomal/lysosomal adaptor, MAPK and MTOR activator 5
• Synonyms gene
  • HBXIP
• Synonyms gene name
  • hepatitis B virus x interacting protein
• Synonyms
  • XIP
  • MGC71071
• Synonyms name
  • HBx-interacting protein
  • hepatitis B virus x-interacting protein (9.6kD)
• GenBank acession
  • AF029890
• Locus
  1p13.3
• Discovery year
  2002-01-10
• Entrez gene record
  10542
• Pubmed identfication
  • 9499022
  • 22980980
• RefSeq identity
  • NM_006402
• Classification
  • Ragulator complex
• VEGA ID
  OTTHUMG00000011568

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