Hemmes Medium Base

Description

Hemmes Medium Base is used to screen and diffrentiate Salmonella and Shigella . The differentiation is based on diffrent seven reactions
Other Product
Alternative Name

NA
Type

Dehydrated Culture Media
Origin

Animal
Classification

Bacterial
Composition g L

Casein enzymic hydrolysate 10.000 Yeast extract 3.000 Dextrose 0.300 Lactose 10.000 Sucrose 10.000 Sodium chloride 4.000 Ferrous sulphate 0.040 Sodium thiosulphate 0.100 Phenol red 0.015 Agar 5.500 Final pH ( at 25°C) 7.2±0.2
Quantity required per litre

42,95 g
Appearance

Light yellow to pink homogeneous free flowing powder
Colour and Clarity of prepared medium

Red coloured, clear to slightly opalescent gel forms in tubes as slants
Cultural response

Cultural characteristics observed with added 40% Urea solution (FD048), after an incubation at 35-37°C for 18-24 hours
Storage

Store below 30°C in tightly closed container and the prepared medium at 2-8°C.
Shelf Life

Use before expiry date on the label.
Technical Data
Data Sheet
Additional description

There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
Group

media

Name

Primary Cell Culture
Concept

Scope note: The initial culturing of cells derived directly from fresh TISSUES.
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data