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Description
Lysine Medium Base is used to isolate and enumerate wild yeasts in pitching yeasts
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Alternative Name
NA
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Type
Dehydrated Culture Media
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Origin
Animal
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Classification
Fungal
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Composition g L
Dextrose 44.500 Monopotassium phosphate 1.780 Magnesium sulphate 0.890 Calcium chloride 0.178 Sodium chloride 0.089 Adenine 0.00178 DL-Methionine 0.000891 L-Histidine 0.000891 DL-Tryptophan 0.000891 Boric acid 0.0000089 Zinc sulphate 0.0000356 Ammonium molybdate 0.0000178 Manganese sulphate 0.0000356 Ferrous sulphate 0.0002225 L-Lysine 1.000 Inositol 0.020 Calcium pantothenate 0.002 Aneurine 0.0004 Pyridoxine 0.0004 p-Amino benzoic acid (PABA) 0.0002 Nicotinic acid 0.0004 Riboflavin 0.0002 Biotin 0.000002 Folic acid 0.000001 Agar 17.800 Final pH ( at 25°C) 5.0±0.2
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Quantity required per litre
66,2 g
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Appearance
White to cream homogeneous free flowing powder
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Colour and Clarity of prepared medium
Colourless to pale yellow clear to slightly opalescent opalescent gel forms in Petri plates
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Cultural response
Cultural characteristics observed after an incubation at 25-30°C upto 7 days.
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Storage
Store below 30°C in tightly closed container and the prepared medium at 2-8°C.
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Shelf Life
Use before expiry date on the label.
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Additional description
There are two major types of growth media those used for cell culture , which use specific cell types derived from plants or animals, and microbiological culture, which are used for growing microorganisms, such as bacteria or yeast. The most common growth media for microorganisms are nutrient broths and agar plates with antibiotics; specialized media are sometimes required for microorganism and cell culture growth.
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Group
media
