Donkey anti Rabbit IgG H + L biotin

• Catalog number
  43C-CB1126
• Price
  Please ask
• Size
  2 mg

• Category
  Secondary Antibody
• Antibody Subtype
  Conjugated Secondary Antibodies
• Area of research
  Immunology
• Type of Immunogen
  Donkey anti-Rabbit IgG (H + L) (biotin) was raised in donkey using purified Rabbit IgG (H&L) ..
• Raised in
  Donkey
• Specificity
  This antibody reacts with heavy (gamma) chains on rabbit IgG and light chains on all rabbit immunoglobulins
• Cross Reactivity
  No reactivity is observed to non-immunoglobulin rabbit serum proteins.
• Conjugate Tag
  Biotin
• Method of Purification
  Affinity purified using solid phase Rabbit IgG (H&L)
• Source
  Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
• Concentration
  1.0 mg/ml (lot specific)
• Form Buffer
  Clear, colorless liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.
• Storage
  Store at 2-8 deg C.
• Shipping conditions
  Blue Ice
• Tested for
  Please refer to the datasheet
• Usage Recommendations
  1:2000-1:20,000 ELISA/WB, 1:500-1:5000 IHC
• URL
• More info
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated
• Latin name
  Equus africanus asinus, Oryctolagus cuniculus
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

• Gene target
  -
• Gene symbol
  CALD1, FUT1, L3MBTL2
• Short name
  anti Rabbit IgG H + L
• Technique
  Rabbit, anti, IgG, antibody to, IgGs
• Host
  Rabbit, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits. Rabbits
• Isotype
  IgG, IgG
• Label
  Biotin
• Species
  Donkeys
• Alternative name
  Donkey anti Rabbit IgG (H + L) secondary antibody (biotin)
• Alternative technique
  rabbit-anti, antibodies, immunoglobulins

Gene info

• Identity
  HGNC:1441
• Gene
  CALD1
• Long gene name
  caldesmon 1
• Synonyms
  • CDM
  • H-CAD
  • L-CAD
• GenBank acession
  • M64110
• Locus
  7q33
• Discovery year
  1992-07-20
• Entrez gene record
  800
• Pubmed identfication
  • 1885618
• RefSeq identity
  • NM_033138
• VEGA ID
  OTTHUMG00000155407

Gene info

• Identity
  HGNC:4012
• Gene
  FUT1
• Long gene name
  fucosyltransferase 1 (H blood group)
• Synonyms gene
  • H
  • HSC
• Synonyms gene name
  • fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase, Bombay phenotype included)
  • fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase)
• Synonyms name
  • galactoside 2-alpha-L-fucosyltransferase
• Locus
  19q13.33
• Discovery year
  1989-06-06
• Entrez gene record
  2523
• RefSeq identity
  • NM_000148
• Classification
  • Fucosyltransferases
  • Blood group antigens
• VEGA ID
  OTTHUMG00000183325
• Locus Specific Databases
  • Blood Group Antigen Mutation Database
  • LRG_810

Gene info

• Identity
  HGNC:18594
• Gene
  L3MBTL2
• Long gene name
  L3MBTL histone methyl-lysine binding protein 2
• Synonyms gene name
  • l(3)mbt-like 2 (Drosophila)
  • L3MBTL2, polycomb repressive complex 1 subunit
• Synonyms
  • H-l(3)mbt-l
  • DKFZP761I141
  • dJ756G23.3
• GenBank acession
  • AJ305226
• Locus
  22q13.2
• Discovery year
  2002-04-29
• Entrez gene record
  83746
• Pubmed identfication
  • 11682070
  • 21596310
• RefSeq identity
  • NM_031488
• Classification
  • MBT domain containing
• VEGA ID
  OTTHUMG00000150942

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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