Donkey anti Sheep IgG H + L biotin

  • Catalog number
    43R-1566
  • Price
    Please ask
  • Size
    2 mg
  • Category
    Secondary Antibody
  • Antibody Subtype
    Conjugated Secondary Antibodies
  • Area of research
    Immunology
  • Type of Immunogen
    Purified Sheep IgG (H&L)
  • Raised in
    Donkey
  • Specificity
    This antibody reacts with heavy (gamma) chains on sheep IgG and light chains on all sheep immunoglobulins.
  • Cross Reactivity
    No reactivity is observed to non-immunoglobulin sheep serum proteins or IgG from human or rabbit.
  • Conjugate Tag
    Biotin
  • Grade Purity
    Grater than 95% pure
  • Method of Purification
    Affinity purified using solid phase Sheep IgG (H&L)
  • Source
    Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
  • Concentration
    1.0 mg/ml (lot specific)
  • Form Buffer
    Clear, colorless liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.
  • Storage
    Store at 2-8 deg C.
  • Shipping conditions
    Blue Ice
  • Tested for
    Please refer to the datasheet
  • Usage Recommendations
    1:2000-1:20,000 ELISA/WB, 1:500-1:5000 IHC
  • URL
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Properties
    Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
  • Conjugation
    Biotinylated
  • Latin name
    Equus africanus asinus
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
  • Gene target
    -
  • Gene symbol
    CALD1, FUT1, L3MBTL2
  • Short name
    anti IgG H + L
  • Technique
    anti, IgG, antibody to, IgGs
  • Host
    Sheep, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits.
  • Isotype
    IgG, IgG
  • Label
    Biotin
  • Species
    Sheep, Donkeys, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
  • Alternative name
    Donkey anti Sheep IgG (H + L) secondary antibody (biotin)
  • Alternative technique
    antibodies, immunoglobulins
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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