Donkey anti Rat IgG H + L biotin
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Catalog number43R-1523
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PricePlease ask
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Size2 mg
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CategorySecondary Antibody
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Antibody SubtypeConjugated Secondary Antibodies
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Area of researchImmunology
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Type of ImmunogenPurified Rat IgG (H&L)
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Raised inDonkey
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SpecificityThis antibody reacts with heavy (gamma) chains on rat IgG and light chains on all rat immunoglobulins.
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Cross ReactivityNo reactivity is observed to non-immunoglobulin rat serum proteins or IgG from bovine, chicken, Goat, Guinea Pig, hamster, horse, human, mouse, rabbit or sheep.
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Conjugate TagBiotin
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Grade PurityGrater than 95% pure
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Method of PurificationAffinity purified using solid phase Rat IgG (H&L)
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SourceDonkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
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Concentration1.0 mg/ml (lot specific)
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Form BufferClear, colorless liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.
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StorageStore at 2-8 deg C.
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Shipping conditionsBlue Ice
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Tested forPlease refer to the datasheet
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Usage Recommendations1:2000-1:20,000 ELISA/WB, 1:500-1:5000 IHC
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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PropertiesBiotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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ConjugationBiotinylated
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Latin nameEquus africanus asinus, Rattus norvegicus
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Gene target-
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Gene symbolCALD1, FUT1, L3MBTL2
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Short nameanti IgG H + L
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Techniqueanti, IgG, antibody to, IgGs
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HostRat, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits.
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IsotypeIgG, IgG
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LabelBiotin
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SpeciesRat, Donkeys, Rats
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Alternative nameDonkey anti Rat IgG (H + L) secondary antibody (biotin)
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Alternative techniqueantibodies, immunoglobulins
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Gene info
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Identity
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Gene
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Long gene namecaldesmon 1
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-07-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namefucosyltransferase 1 (H blood group)
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Synonyms gene
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Synonyms gene name
- fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase, Bombay phenotype included)
- fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase)
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Synonyms name
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Locus
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Discovery year1989-06-06
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Entrez gene record
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RefSeq identity
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Classification
- Fucosyltransferases
- Blood group antigens
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameL3MBTL histone methyl-lysine binding protein 2
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Synonyms gene name
- l(3)mbt-like 2 (Drosophila)
- L3MBTL2, polycomb repressive complex 1 subunit
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Synonyms
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GenBank acession
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Locus
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Discovery year2002-04-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MBT domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data