Donkey anti Rabbit IgG H + L biotin
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Catalog number
43R-1442
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Price
Please ask
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Size
2 mg
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Category
Secondary Antibody
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Antibody Subtype
Conjugated Secondary Antibodies
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Area of research
Immunology
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Type of Immunogen
Purified Rabbit IgG (H&L)
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Raised in
Donkey
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Specificity
This antibody reacts with heavy (gamma) chains on rabbit IgG and light chains on all rabbit immunoglobulins.
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Cross Reactivity
No reactivity is observed to non-immunoglobulin rabbit serum proteins, mouse IgG or serum proteins.
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Conjugate Tag
Biotin
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Grade Purity
Grater than 95% pure
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Method of Purification
Affinity purified using solid phase Rabbit IgG (H&L)
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Source
Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
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Concentration
1.0 mg/ml (lot specific)
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Form Buffer
Clear, colorless liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.
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Storage
Store at 2-8 deg C.
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Shipping conditions
Blue Ice
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Tested for
Please refer to the datasheet
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Usage Recommendations
1:2000-1:20,000 ELISA/WB, 1:500-1:5000 IHC
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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Latin name
Equus africanus asinus, Oryctolagus cuniculus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Gene target
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Gene symbol
CALD1, FUT1, L3MBTL2
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Short name
anti Rabbit IgG H + L
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Technique
Rabbit, anti, IgG, antibody to, IgGs
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Host
Rabbit, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits. Rabbits
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Isotype
IgG, IgG
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Label
Biotin
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Species
Donkeys
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Alternative name
Donkey anti Rabbit IgG (H + L) secondary antibody (biotin)
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Alternative technique
rabbit-anti, antibodies, immunoglobulins
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Gene info
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Identity
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Gene
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Long gene name
caldesmon 1
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Synonyms
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GenBank acession
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Locus
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Discovery year
1992-07-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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