Biotin Antibody Fab - FITC Conjugated
#
-
Catalog numberOARA01747
-
Price:
-
Sizeone vial
-
-
Recognized antigenBiotin Fab
-
Product typeAntibody
-
Gene nameBiotin Fab
-
Long nameAntibody against Biotin Fab
-
StorageFor shorter time periods the Biotin Fab Antibodies may be kept refrigerated at +4 degrees Celsius. For extended periods of time keep frozen at -20 °C. Avoid freezing and thawing the lysate repeatedly as these cycles will cause denaturation and/or disruption of the protein chains and peptide bonds of the Biotin Fab Antibodies which might cause change or loss of the desired properties.
-
TipsSmall amounts of the Biotin Fab antibody may get entrapped on the walls or lid of the vial as a result of shaking during transportation or handling. Prior to use, cetrifuge the vial to ensure that all of its content is collected on the bottom. In order to avoid cycles of freezing and thawing we recommend to plan and prepare aliquots according to the experiment demand and defrost only the amount which wil be needed for immediate use.
-
PropertiesIf you buy Antibodies supplied by aviva they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Human Fab Of Normal IgG Chromatographically purified Fab fragment of human IgG. Purified human Fab may be used as a reference antigen, calibrator, coating protein and blocking agent, This aviva Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. aviva FITC is highly sensitive to pH extremes.
-
ConjugationBiotinylated, Anti-FITC Antibody
-
French translationanticorps
-
Gene target
-
Short nameAnti-Biotin Fab
-
TechniqueAntibody, FITC, Fab, antibodies against human proteins, antibodies for, antibody Conjugates, fabs, Fluorescein
-
IsotypeFab fragment
-
LabelBiotin
-
Alternative nameBiotin Fab Antibodies
-
Alternative techniqueantibodies, fluorescine, seconairy
-
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data