CD25 / IL-2R alpha chain monoclonal antibody

• Catalog number
  11-218-C025
• Price
  Please ask
• Size
  25ug

• Long name
  Mouse Monoclonal Antibody against Human CD25 / IL-2R alpha chain, Clone: MEM-181
• Antigen
  CD25
• Species reactivity
  Human (Homo sapiens)
• Negative species reactivity
  at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
• Clonality
  Monoclonal Antibody (Mab)
• Clone
  MEM-181
• Applications
  FC
• Immunogen
  PHA-activated peripheral blood leucocytes
• Format conjugation
  purified; unconjugated
• Specificity
  The antibody MEM-181 reacts with CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes._x000D_ HLDA VI; WS Code NL N-L024 _x000D_
• Research area
  CD and Related Antigens (Human)
• Concentration
  1 mg/ml
• Storage buffer
  Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
• Shipping and storage
  Mouse Monoclonal Antibody against Human CD25 / IL-2R alpha chain, Clone: MEM-181 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
• Background
  CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas.
• Purity
  > 95% (by SDS-PAGE)
• Purification method
  Purified by protein-A affinity chromatography
• Datasheet
• References
  *Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84., *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
• Description
  The Anti-CD25 / IL-2R alpha chain Mab is a α- or alpha protein sometimes glycoprotein present in blood.
• Properties
  If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  CD25   IL-2R   alpha   chain   monoclonal  
• Gene symbol
  IL2RA, VCX2, ISG20, IL36G, IL25, IL22, IL18, IL37, IL6R, IL17C
• Short name
  Anti-CD25 / IL-2R alpha chain Mab
• Technique
  Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
• Host
  Mouse (Mus musculus)
• Isotype
  IgG1
• Alternative name
  Mouse Monoclonal to CD25 / IL-2R alpha chain
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:6008
• Gene
  IL2RA
• Long gene name
  interleukin 2 receptor subunit alpha
• Synonyms gene
  • IL2R
  • IDDM10
• Synonyms gene name
  • insulin-dependent diabetes mellitus 10
  • interleukin 2 receptor, alpha
• Synonyms
  • CD25
• GenBank acession
  • X01057
• Locus
  10p15.1
• Discovery year
  1990-01-22
• Entrez gene record
  3559
• Pubmed identfication
  • 3925551
  • 17676041
• RefSeq identity
  • NM_000417
• Classification
  • CD molecules
  • Sushi domain containing
  • Interleukin receptors
• VEGA ID
  OTTHUMG00000017616
• Locus Specific Databases
  • IL2RAbase: Mutation registry for Interleuken 2 receptor α deficiency
  • LRG_73

Gene info

• Identity
  HGNC:18158
• Gene
  VCX2
• Long gene name
  variable charge X-linked 2
• Synonyms
  • VCX-2r
  • VCX-2R
• GenBank acession
  • AF159127
• Locus
  Xp22.31
• Discovery year
  2004-04-16
• Entrez gene record
  51480
• Pubmed identfication
  • 10607842
• RefSeq identity
  • NM_016378
• Classification
  • Variable charge X/Y family
• VEGA ID
  OTTHUMG00000021105

Gene info

• Identity
  HGNC:6130
• Gene
  ISG20
• Long gene name
  interferon stimulated exonuclease gene 20
• Synonyms gene name
  • interferon stimulated exonuclease gene 20kDa
• Synonyms
  • HEM45
  • CD25
• GenBank acession
  • X89773
• Locus
  15q26.1
• Discovery year
  1998-01-30
• Entrez gene record
  3669
• Pubmed identfication
  • 9235947
  • 9605874
• RefSeq identity
  • NM_002201
• Classification
  • Exonucleases
• VEGA ID
  OTTHUMG00000148679

Gene info

• Identity
  HGNC:15741
• Gene
  IL36G
• Long gene name
  interleukin 36 gamma
• Synonyms gene
  • IL1F9
• Synonyms gene name
  • interleukin 1 family, member 9
• Synonyms
  • IL-1H1
  • IL-1RP2
  • IL-1F9
  • IL1H1
  • IL1E
• Synonyms name
  • interleukin-1 homolog 1
  • interleukin 1-related protein 2
  • interleukin-1 epsilon
• GenBank acession
  • AF200492
• Locus
  2q14.1
• Discovery year
  2002-08-02
• Entrez gene record
  56300
• Pubmed identfication
  • 10860666
  • 10744718
  • 11991722
  • 11991723
• RefSeq identity
  • NM_019618
• Classification
  • Interleukins
• VEGA ID
  OTTHUMG00000131336

Gene info

• Identity
  HGNC:13765
• Gene
  IL25
• Long gene name
  interleukin 25
• Synonyms gene
  • IL17E
• Synonyms gene name
  • interleukin 17E
• Synonyms
  • IL-25
  • IL-17E
• GenBank acession
  • AF305200
• Locus
  14q11.2
• Discovery year
  2001-01-26
• Pubmed identfication
  • 11058597
  • 11754819
• Classification
  • Interleukins
• VEGA ID
  OTTHUMG00000028749

Gene info

• Identity
  HGNC:14900
• Gene
  IL22
• Long gene name
  interleukin 22
• Synonyms
  • ILTIF
  • IL-21
  • zcyto18
  • IL-TIF
  • IL-D110
  • TIFa
  • TIFIL-23
  • IL-22
  • MGC79382
  • MGC79384
• Synonyms name
  • IL-10-related T-cell-derived inducible factor
• GenBank acession
  • AF279437
• Locus
  12q15
• Discovery year
  2001-03-12
• Entrez gene record
  50616
• Pubmed identfication
  • 10954742
  • 10875937
• RefSeq identity
  • NM_020525
• Classification
  • Interleukins
• VEGA ID
  OTTHUMG00000169119
• Locus Specific Databases
  • LRG_1248

Gene info

• Identity
  HGNC:5986
• Gene
  IL18
• Long gene name
  interleukin 18
• Synonyms gene name
  • interleukin 18 (interferon-gamma-inducing factor)
• Synonyms
  • IGIF
  • IL1F4
  • IL-1g
  • IL-18
• Synonyms name
  • interferon-gamma-inducing factor
• GenBank acession
  • U90434
• Locus
  11q23.1
• Discovery year
  1997-07-25
• Entrez gene record
  3606
• Pubmed identfication
  • 7477296
  • 9693051
• RefSeq identity
  • NM_001562
• Classification
  • Interleukins
• VEGA ID
  OTTHUMG00000167006

Gene info

• Identity
  HGNC:15563
• Gene
  IL37
• Long gene name
  interleukin 37
• Synonyms gene
  • IL1F7
• Synonyms gene name
  • interleukin 1 family, member 7 (zeta)
• Synonyms
  • FIL1
  • FIL1Z
  • FIL1(ZETA)
  • IL-1H4
  • IL-1RP1
  • IL-1F7
• Synonyms name
  • interleukin 1, zeta
  • interleukin-1 homolog 4
  • interleukin-1-related protein
• GenBank acession
  • AF201832
• Locus
  2q14.1
• Discovery year
  2002-08-02
• Entrez gene record
  27178
• Pubmed identfication
  • 10625660
  • 10512743
  • 12496389
• RefSeq identity
  • NM_014439
• Classification
  • Interleukins
• VEGA ID
  OTTHUMG00000131345

Gene info

• Identity
  HGNC:6019
• Gene
  IL6R
• Long gene name
  interleukin 6 receptor
• Synonyms
  • CD126
  • IL-6R
  • IL-1Ra
  • IL6RA
  • gp80
• Synonyms name
  • membrane glycoprotein 80
  • interleukin 6 receptor subunit alpha
• GenBank acession
  • X12830
• Locus
  1q21.3
• Discovery year
  2001-06-22
• Entrez gene record
  3570
• Pubmed identfication
  • 8428753
  • 12633863
• RefSeq identity
  • NM_000565
• Classification
  • CD molecules
  • Interleukin receptors
  • Immunoglobulin like domain containing
• VEGA ID
  OTTHUMG00000036073

Gene info

• Identity
  HGNC:5983
• Gene
  IL17C
• Long gene name
  interleukin 17C
• Synonyms
  • IL-17C
  • CX2
  • IL-21
  • MGC126884
  • MGC138401
• GenBank acession
  • AF152099
• Locus
  16q24.2
• Discovery year
  2000-01-20
• Entrez gene record
  27189
• Pubmed identfication
  • 10639155
• RefSeq identity
  • NM_013278
• Classification
  • Interleukins
• VEGA ID
  OTTHUMG00000173241

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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