M13 bacteriophage (g3p) Biotinylated, Monoclonal Antibody
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Catalog number
Z110M
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Price
Please ask
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Size
100µg
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Host Source
Mouse
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Clone Name
E1
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Product Category
Molecular Biology
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Species Reactivity
N/A
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Application
ELISA, Western Blot, Flow Cytometry, IHC
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Immunogen
Hybridoma produced by the fusion of splenocytes from mice immunized with isolated M13 phage coat proteins and mouse myeloma cells.
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Purification Method
Protein A/G Chromatography
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Shipping Conditions
Ship at ambient temperature, freeze upon arrival
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Storage conditions
M13 bacteriophage (g3p) Biotinylated, Monoclonal Antibody should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
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Properties
If you buy Antibodies supplied by Exalpha they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Gene
M13 bacteriophage is a virus that infects the bacterium Escherichia coli. It is composed of a circular single-stranded DNA molecule encased in a thin flexible tube made up of about 2700 copies of a single protein called P8, the major coat protein. The ends of the tube are capped with minor coat proteins. Infection starts when the minor coat protein P3 attaches to the receptor at the tip of the F pilus of the bacterium. Infection with M13 is not lethal; however, the infection causes turbid plaques in E. coli because infected bacteria grow more slowly than the surrounding uninfected bacteria. It engages in a viral lifestyle known as a chronic infection which is neither lytic or temperate. However a decrease in the rate of cell growth is seen in the infected cells. M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures and nanotechnology.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
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Gene target
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Short name
M13 bacteriophage (g3p) Biotinylated, Monoclonal Antibody
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Technique
Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
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Isotype
IgG2a
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Alternative name
M13 bacteriophage (g3p) Biotinylated, monoclonal (antibody to-)
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Alternative technique
antibodies
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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