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Products_type
Antibody
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Products_short_name
[M13 bacteriophage]
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Products_name_syn
[Coat protein A; g3p; Minor coat protein]
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Other_names
[structural protein; Attachment protein G3P; attachment protein; Gene 3 protein; G3P; Minor coat protein]
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Other_gene_names
[III; III; G3P]
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Clonality
Monoclonal
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Clone
[E1]
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Purity
Protein A/G Chromatography
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Form
Provided as solution in phosphate buffered saline with 0.08% sodium azide
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Storage_stability
Store at -20 degree C. Aliquot to avoid freeze/thaw cycles
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Tested_application
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
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Gene
M13 bacteriophage is a virus that infects the bacterium Escherichia coli. It is composed of a circular single-stranded DNA molecule encased in a thin flexible tube made up of about 2700 copies of a single protein called P8, the major coat protein. The ends of the tube are capped with minor coat proteins. Infection starts when the minor coat protein P3 attaches to the receptor at the tip of the F pilus of the bacterium. Infection with M13 is not lethal; however, the infection causes turbid plaques in E. coli because infected bacteria grow more slowly than the surrounding uninfected bacteria. It engages in a viral lifestyle known as a chronic infection which is neither lytic or temperate. However a decrease in the rate of cell growth is seen in the infected cells. M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures and nanotechnology.
