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Products_type
Antibody
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Products_short_name
[M13 Bacteriophage]
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Products_name_syn
[Anti -M13 Bacteriophage]
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Clonality
Monoclonal
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Clone
[10F25]
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Specificity
Recognizes the gp3 phage coat protein of the M13 bacteriophage, widely used in the preparation of 'phage-display' immunoglobulin libraries.
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Purity
Purified Purified
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Form
Supplied as a liquid in PBS, 0.09% sodium azide.
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Storage_stability
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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Tested_application
ELISA (EL/EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
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Gene
M13 bacteriophage is a virus that infects the bacterium Escherichia coli. It is composed of a circular single-stranded DNA molecule encased in a thin flexible tube made up of about 2700 copies of a single protein called P8, the major coat protein. The ends of the tube are capped with minor coat proteins. Infection starts when the minor coat protein P3 attaches to the receptor at the tip of the F pilus of the bacterium. Infection with M13 is not lethal; however, the infection causes turbid plaques in E. coli because infected bacteria grow more slowly than the surrounding uninfected bacteria. It engages in a viral lifestyle known as a chronic infection which is neither lytic or temperate. However a decrease in the rate of cell growth is seen in the infected cells. M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures and nanotechnology.
