M13 Bacteriophage (Biotin)
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Catalog number
MBS602902
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Price
Please ask
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Size
5x0.1 mg
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Products_type
Antibody
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Products_short_name
[M13 Bacteriophage]
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Products_name_syn
[Anti -M13 Bacteriophage (Biotin)]
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Clonality
Monoclonal
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Clone
[3H2610]
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Specificity
Recognizes the M13 bacteriophage. Detects binding of ScFv antibodies developed in the M13 system.
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Purity
Purified Purified
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Form
Supplied as a liquid in PBS, 0.08% sodium azide, before the addition of glycerol to 40%. Labeled with Biotin.
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Storage_stability
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested_application
ELISA (EL/EIA), Flow Cytometry (FC/FACS)
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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Gene
M13 bacteriophage is a virus that infects the bacterium Escherichia coli. It is composed of a circular single-stranded DNA molecule encased in a thin flexible tube made up of about 2700 copies of a single protein called P8, the major coat protein. The ends of the tube are capped with minor coat proteins. Infection starts when the minor coat protein P3 attaches to the receptor at the tip of the F pilus of the bacterium. Infection with M13 is not lethal; however, the infection causes turbid plaques in E. coli because infected bacteria grow more slowly than the surrounding uninfected bacteria. It engages in a viral lifestyle known as a chronic infection which is neither lytic or temperate. However a decrease in the rate of cell growth is seen in the infected cells. M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures and nanotechnology.
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