FLAG Tag (1557CT661.18.1) Monoclonal Antibody
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Catalog number
bsm-51321M
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Price
Please ask
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Size
0.1ml
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Long name
FLAG Tag (1557CT661.18.1) Monoclonal Antibody
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Also known as
FLAG Tag (1557CT661.18.1) Monoclonal Antibody
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Category
Primary Antibodies
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Conjugation
Unconjugated
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Target Antigen
FLAG Tag
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Specificity
This is a highly specific antibody against FLAG Tag.
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Modification s
None
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Modification site s
Unmodified antibody
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Clonality
Monoclonal
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Clone number
1557CT661.18.1
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Source
KLH conjugated synthetic peptide encoding HA tag (DYKDDDDKC) was used as antigen.
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Tested Applications
WB
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Recommended dilutions
WB(1:2000)
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Cross reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Storage conditions
Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
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Properties
If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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Additional conjugation
Flag
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
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Gene target
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Short name
Anti-FLAG Tag
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Technique
Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, Tags
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Host
Mouse (Mus musculus)
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Isotype
Immunoglobulin G (IgG)1 κ
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Label
Flag
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Alternative name
FLAG Tag (1557CT661.18.1) Monoclonal Antibody
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Alternative technique
antibodies
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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