FITC Antibody
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Catalog numberA57819-100
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PricePlease ask
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Size0.1mg
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Product categoryAntibodies
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ConjugationUnconjugated
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ClonalityPolyclonal antibody
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Target antigenFITC
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Long nameFITC Polyclonal Antibody
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StorageFor long term storage keep frozen at -20 deg.C.
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TipsFor storage over extended periods of time we recommend you to prepare aliquotes of the FITC Antibody and freeze the. Afterwards thaw only the vial(s) you intend to use. This way you will avoid the freeze-thaw cycles. Due to shipping and handling issues small amounts of the antibodies may get trapped on the lid and walls of the tube. Prior to use briefly centrifuge the vials to ensure that all of the content is on the bottom.
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PropertiesIf you buy Antibodies supplied by Epigen they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Epigen Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Epigen FITC is highly sensitive to pH extremes.
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Additional conjugationAnti-FITC Antibody
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French translationanticorps
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Short nameAnti-FITC
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TechniqueAntibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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LabelFITC
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Alternative nameFITC PAb
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Alternative techniqueantibodies, fluorescine
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data