Recombinant Human PDE4B / DPDE4 Protein (His & GST tag) 50µg

• Catalog number
  PKSH031100-50µg
• Price
  Please ask
• Size
  50µg

• Synonym
  DPDE4;PDEIVB
• Activity
  NA
• Sequence
  Met 1-Thr 564
• Fusion tag
  N-His & GST
• Accession
  NP_001032416.1
• Expressed Host
  Baculovirus-Insect Cells
• Shipping
  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Purity
  ＞80 % as determined by SDS-PAGE
• Endotoxin
  ＜1.0 EU per µg as determined by the LAL method
• Stability and Storage
  Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Mol Mass
  92.2 kDa
• AP Mol Mass
  100 kDa
• Formulation
  Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM GSH, 10% gly, 0.5mM PMSF, pH 8.0
• Reconstitution
  Please refer to it for detailed information.
• Background
  cAMP-specific 3',5'-cyclic phosphodiesterase 4B, also known as PDE4B and DPDE4, is a member of the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. Cyclic nucleotide phosphodiesterases (PDEs) comprise a large family of enzymes that catalyze the hydrolysis of cAMP or cGMP and are implicated in various diseases. The crystal structures reveal a common scheme of inhibitor binding to the PDEs: (i) a hydrophobic clamp formed by highly conserved hydrophobic residues that sandwich the inhibitor in the active site; (ii) hydrogen bonding to an invariant glutamine that controls the orientation of inhibitor binding. A scaffold can be readily identified for any given inhibitor based on the formation of these two types of conserved interactions. These structural insights will enable the design of isoform-selective inhibitors with improved binding affinity and should facilitate the discovery of more potent and selective PDE inhibitors for the treatment of a variety of diseases. PDE4B / DPDE4 hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. It is expressed in brain, heart, lung and skeletal muscle. PDE4B / DPDE4 may be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents
• Product URL
• PDF URL
• Conjugation
  histidine
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  PDE4B   DPDE4   Protein   His   GST   tag   50µg  
• Gene symbol
  PDE4B, PDE4B-AS1, MGST3
• Short name
  Recombinant PDE4B / DPDE4 Protein (His & GST tag) 50µg
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  His
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens PDE4B / DPDE4 Protein (histidine & GST detection labelled) 50µg
• Alternative technique
  rec

Gene info

• Identity
  HGNC:8781
• Gene
  PDE4B
• Long gene name
  phosphodiesterase 4B
• Synonyms gene
  • DPDE4
• Synonyms gene name
  • phosphodiesterase 4B, cAMP-specific (dunce (Drosophila)-homolog phosphodiesterase E4)
  • phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila)
  • phosphodiesterase 4B, cAMP-specific
• Synonyms name
  • phosphodiesterase E4 dunce homolog (Drosophila)
  • cAMP-specific 3',5'-cyclic phosphodiesterase 4B
• GenBank acession
  • L20971
• Locus
  1p31.3
• Discovery year
  1994-07-29
• Entrez gene record
  5142
• RefSeq identity
  • NM_002600
• Classification
  • Phosphodiesterases
• VEGA ID
  OTTHUMG00000009088

Gene info

• Identity
  HGNC:40437
• Gene
  PDE4B-AS1
• Long gene name
  PDE4B antisense RNA 1
• Locus
  1p31.3
• Discovery year
  2019-09-19
• Entrez gene record
  101927139
• RefSeq identity
  • NR_123718
• Classification
  • Antisense RNAs

Gene info

• Identity
  HGNC:7064
• Gene
  MGST3
• Long gene name
  microsomal glutathione S-transferase 3
• Synonyms
  • GST-III
• Synonyms name
  • microsomal glutathione S-transferase III
  • microsomal GST-3
  • microsomal GST-III
• GenBank acession
  • AF026977
• Locus
  1q24.1
• Discovery year
  1997-10-16
• Entrez gene record
  4259
• Pubmed identfication
  • 9278457
• RefSeq identity
  • NM_004528
• Classification
  • Microsomal glutathione S-transferases
• VEGA ID
  OTTHUMG00000034627

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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