Recombinant Human EphB3 / HEK2 Protein (aa 585-998, His & GST tag) 50µg
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Catalog numberPKSH030324-50µg
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PricePlease ask
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Size50µg
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SynonymETK2;HEK2;TYRO6
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ActivityThe specific activity was determined to be 90 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.
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SequenceGln585-Val998
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Fusion tagN-His & GST
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AccessionP54753
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Expressed HostBaculovirus-Insect Cells
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ShippingKinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
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Purity>90 % as determined by SDS-PAGE
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Endotoxin<1.0 EU per µg as determined by the LAL method
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Stability and StorageSamples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass74.7 kDa
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AP Mol Mass64 kDa
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FormulationSupplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly
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ReconstitutionPlease refer to it for detailed information.
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BackgroundEphrin type-B receptor 3, also known as EphB3 or HEK2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.
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Conjugationhistidine
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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SourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolMGST3, EPHB3, MIR585
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Short nameRecombinant EphB3 / HEK2 Protein (aa 585-998, His & GST tag) 50µg
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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LabelHis
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SpeciesHuman, Humans
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Alternative nameRec. H. sapiens EphB3 / HEK2 Protein (aa 585-998, histidine & GST detection labelled) 50µg
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene namemicrosomal glutathione S-transferase 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-10-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Microsomal glutathione S-transferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameEPH receptor B3
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Synonyms gene
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Synonyms gene name
- EphB3
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-12-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Sterile alpha motif domain containing
- EPH receptors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 585
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Synonyms gene
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Synonyms
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Locus
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Discovery year2006-05-16
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNAs
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data