Recombinant Human DCAMKL1 / DCLK1 Protein (aa 1-705, His & GST tag) 50µg
-
Catalog numberPKSH030359-50µg
-
PricePlease ask
-
Size50µg
-
-
SynonymCL1;CLICK1;DCAMKL1;DCDC3A;DCLK
-
ActivityThe specific activity was determined to be 6.1 nmol/min/mg using synthetic Autocamtide-2 peptide (KKALRRQETVDAL-amide) as substrate.
-
SequenceMet 1-Val 705
-
Fusion tagN-His & GST
-
AccessionO15075-1
-
Expressed HostBaculovirus-Insect Cells
-
ShippingKinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
-
Purity>84 % as determined by SDS-PAGE
-
Endotoxin<1.0 EU per µg as determined by the LAL method
-
Stability and StorageSamples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Mol Mass106 kDa
-
AP Mol Mass105 kDa
-
FormulationSupplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly, 0.5mM PMSF
-
ReconstitutionPlease refer to it for detailed information.
-
BackgroundDCAMKL1, also known as DCLK1, is a member of the protein kinase superfamily and the doublecortin family. It contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. DCAMKL1 is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. Its microtubule-polymerizing activity is independent of its protein kinase activity. DCAMKL1 may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. It may also participate in functions of the mature nervous system.
-
Conjugationhistidine
-
PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
SourceRecombinants or rec. proteins
-
Grouprecombinants
-
Gene target
-
Gene symbolDCLK1, MGST3, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR1289-1, MIR101-1, MIR16-1
-
Short nameRecombinant DCAMKL1 / DCLK1 Protein (aa 1-705, His & GST tag) 50µg
-
TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
-
LabelHis
-
SpeciesHuman, Humans
-
Alternative nameRec. H. sapiens DCAMKL1 / DCLK1 Protein (aa 1-705, histidine & GST detection labelled) 50µg
-
Alternative techniquerec
-
Gene info
-
Identity
-
Gene
-
Long gene namedoublecortin like kinase 1
-
Synonyms gene
-
Synonyms gene name
- doublecortin and CaM kinase-like 1
- doublecortin-like kinase 1
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1999-02-22
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Doublecortin superfamily
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namemicrosomal glutathione S-transferase 3
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1997-10-16
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Microsomal glutathione S-transferases
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin heavy variable (II)-1-1 (pseudogene)
-
Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2000-04-17
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin heavy locus at 14q32.33
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namemicroRNA 1-1
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2004-04-23
-
Entrez gene record
-
RefSeq identity
-
Classification
- MicroRNA MIR1/206 family
Gene info
-
Identity
-
Gene
-
Long gene nametRNA-undetermined (NNN) 8-1
-
Synonyms gene name
- transfer RNA-undetermined (NNN) 8-1
-
GenBank acession
-
Locus
-
Discovery year2014-06-20
-
Entrez gene record
-
Pubmed identfication
Gene info
-
Identity
-
Gene
-
Long gene nametRNA-undetermined (NNN) 7-1
-
Synonyms gene name
- transfer RNA-undetermined (NNN) 7-1
-
GenBank acession
-
Locus
-
Discovery year2014-06-20
-
Entrez gene record
-
Pubmed identfication
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin kappa variable 1/OR2-1 (pseudogene)
-
Synonyms gene
-
Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2000-04-18
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Immunoglobulin kappa (IGK) orphons
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namemicroRNA 1289-1
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2008-11-03
-
Entrez gene record
-
RefSeq identity
-
Classification
- MicroRNA MIR1289 family
Gene info
-
Identity
-
Gene
-
Long gene namemicroRNA 101-1
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2004-04-23
-
Entrez gene record
-
RefSeq identity
-
Classification
- MicroRNA MIR101 family
Gene info
-
Identity
-
Gene
-
Long gene namemicroRNA 16-1
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2004-04-23
-
Entrez gene record
-
RefSeq identity
-
Classification
- MicroRNA MIR15/16 family
MeSH Data
-
Name
-
ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
-
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data