Affinity purified Hamster IgG Isotype Antibody FITC-Conjugated

• Catalog number
  EHIS002-50
• Price
  Please ask
• Size
  50 mg

• Clone
  Polyclonal
• Source
  Hamster
• Clonality
  Polyclonal
• Category
  Isotype Control
• Description
  The isotype of this antibody is Hamster IgG. This hamster IgG fraction was purified by affinity chromatography from Armenian hamster serum.
• Reactivity
  Not specified / Inquire
• Conjugation
  FITC
• Purification
  Affinity purified
• Antibody s supplied with
  PBS pH 7.2, 0.09% NaN3, 0.2% BSA
• Antibody s concentration
  0,5 mg/ml
• Storage
  Keep as concentrated solution. Store at 4°C and protected from prolonged exposure to light. Do not freeze.
• Antibody s is used for
  Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis (The amount of the reagent is suggested to be used ≤ 0.5 µg /106 cells in 100 µl). Since applications vary, the appropriate dilutions must be determined for individual use.
• Specifications
• Test
  A high affinity purification column was use to purify IgG Isotype Antibody FITC-Conjugated by Elabscience by chromatographic size exclusion.
• Additional description
  1 of protein A or G purified by Elabscience will give more specificity than crude serum.
• Properties
  If you buy Antibodies supplied by Elabscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Elabscience Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Elabscience FITC is highly sensitive to pH extremes. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
• Additional conjugation
  Anti-FITC Antibody
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
• Group
  isotypes
• French translation
  anticorps

• Gene target
  Conjugated  
• Short name
  IgG Isotype Antibody FITC-Conjugated
• Technique
  affinity, Antibody, purified, Isotype, FITC, IgG, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, IgGs, Isotypes and controls, purifications
• Host
  Hamster
• Isotype
  IgG, IgG
• Label
  FITC
• Species
  Hamster, Hamsters
• Alternative name
  protein enriched Hamster Immunoglobulin G isoform (antibody to-) fluorecein-coupled
• Alternative technique
  pure, antibodies, control, fluorescine, immunoglobulins

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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