IgG Isotype Control Fc fusion protein FITC
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Catalog number35R-0013
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Price:
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Size50 µg
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CategoryBiological Reagents
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Antibody SubtypeIsotype Controls
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Area of researchImmunology
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Raised inHamster
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IsotypesIgG
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Concentration500 ug/ml
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Form BufferProvided in aqueous buffer with 0.09% NaN3, may contain carrier protein/stabilizer.
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StorageStore at 2-8 deg C. Do not freeze. Protect from light.
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Shipping conditionsBlue Ice
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Tested forFC
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Usage RecommendationsFC: ≤ 1 ug/test
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Assay InformationA test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
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Additional InformationThis antibody is offered in 2 formats.
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DescriptionIsotype or positive controls by peptides, antibodies and deactivated samples. Fusion proteins or chimeric proteins are proteins created through the joining of two or more genes that originally coded for separate proteins. A GFP gene is often used as tag to a reporter gene. Fusion lentiverctors can be used as viral particles to produce proteins that carry for example a GFP tag. Antigen purification of recombinant fusion tag proteins is a frequent strategy using a Fralg tag.
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PropertiesThis fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
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ConjugationAnti-FITC Antibody
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Groupisotypes
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Gene target
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Gene symbolFCGBP, FCRL2
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Short nameIgG Isotype Control Fc protein FITC
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TechniqueControl, Isotype, FITC, IgG, FC, controls, Fluorescein, IgGs, Isotypes and controls
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IsotypeIgG, IgG
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LabelFITC
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Alternative nameSyrian Hamster monoclonal IgG Isotype Control Fc fusion protein (FITC)
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Alternative techniquecontrol, controls, fluorescine, immunoglobulins
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Tissuecontrol
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Gene info
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Identity
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Gene
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Long gene nameFc fragment of IgG binding protein
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-09-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameFc receptor like 2
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Synonyms gene
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Synonyms gene name
- SH2 domain-containing phosphatase anchor protein 1
- Fc receptor-like 2
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-04-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Immunoglobulin like domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data