Affinity purified Hamster IgG Isotype Antibody BIOTIN-Conjugated
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Catalog number
EHIS001-200
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Price
Please ask
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Size
200 mg
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Clone
Polyclonal
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Source
Hamster
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Clonality
Polyclonal
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Category
Isotype Control
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Description
The isotype of this antibody is Hamster IgG. This hamster IgG fraction was purified by affinity chromatography from Armenian hamster serum.
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Reactivity
Not specified / Inquire
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Conjugation
BIOTIN
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Purification
Affinity purified
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Antibody s supplied with
PBS pH 7.2, 0.09% NaN3, 0.2% BSA
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Antibody s concentration
0,5 mg/ml
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Storage
Keep as concentrated solution. Store at 4°C and protected from prolonged exposure to light. Do not freeze.
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Antibody s is used for
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis (The amount of the reagent is suggested to be used ≤ 0.5 µg /106 cells in 100 µl). Since applications vary, the appropriate dilutions must be determined for individual use.
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Test
A high affinity purification column was use to purify IgG Isotype Antibody -Conjugated by Elabscience by chromatographic size exclusion.
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Additional description
1 of protein A or G purified by Elabscience will give more specificity than crude serum.
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Properties
If you buy Antibodies supplied by Elabscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
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Additional conjugation
Biotinylated
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Group
isotypes
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French translation
anticorps
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Gene target
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Short name
IgG Isotype Antibody -Conjugated
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Technique
affinity, Antibody, purified, Isotype, IgG, antibodies against human proteins, antibodies for, antibody Conjugates, IgGs, Isotypes and controls, purifications
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Host
Hamster
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Isotype
IgG, IgG
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Label
Biotin
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Species
Hamster, Hamsters
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Alternative name
protein enriched Hamster Immunoglobulin G isoform (antibody to-) biotinilated-coupled
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Alternative technique
pure, antibodies, control, immunoglobulins
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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