Affinity purified Hamster IgG Isotype Antibody BIOTIN-Conjugated
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Catalog numberEHIS001-50
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Price:
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Size50 mg
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ClonePolyclonal
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SourceHamster
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ClonalityPolyclonal
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CategoryIsotype Control
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DescriptionThe isotype of this antibody is Hamster IgG. This hamster IgG fraction was purified by affinity chromatography from Armenian hamster serum.
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ReactivityNot specified / Inquire
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ConjugationBIOTIN
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PurificationAffinity purified
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Antibody s supplied withPBS pH 7.2, 0.09% NaN3, 0.2% BSA
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Antibody s concentration0,5 mg/ml
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StorageKeep as concentrated solution. Store at 4°C and protected from prolonged exposure to light. Do not freeze.
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Antibody s is used forEach lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis (The amount of the reagent is suggested to be used ≤ 0.5 µg /106 cells in 100 µl). Since applications vary, the appropriate dilutions must be determined for individual use.
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TestA high affinity purification column was use to purify IgG Isotype Antibody -Conjugated by Elabscience by chromatographic size exclusion.
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Additional description1 of protein A or G purified by Elabscience will give more specificity than crude serum.
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PropertiesIf you buy Antibodies supplied by Elabscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
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Additional conjugationBiotinylated
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Groupisotypes
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French translationanticorps
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Gene target
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Short nameIgG Isotype Antibody -Conjugated
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Techniqueaffinity, Antibody, purified, Isotype, IgG, antibodies against human proteins, antibodies for, antibody Conjugates, IgGs, Isotypes and controls, purifications
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HostHamster
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IsotypeIgG, IgG
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LabelBiotin
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SpeciesHamster, Hamsters
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Alternative nameprotein enriched Hamster Immunoglobulin G isoform (antibody to-) biotinilated-coupled
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Alternative techniquepure, antibodies, control, immunoglobulins
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data