Soluble Epoxide Hydrolase Activity Assay Kit (Fluorometric)

• Catalog number
  K477-100
• Price
  Please ask
• Size
  100 assays

• Description
  Highly Sensitive Assay for the detection of Soluble Epoxide Hydrolase; Includes sEH Positive Control
• Summary
  • Detection method-Fluorescence (Ex/Em: 362/460 nm) • Applications- Measurement of sEH activity in cell and tissue lysates using a 96-well plate format
• Detection Method
  Fluorescence (Ex/Em: 362/460 nm)
• Species Reactivity
  Eukaryotes
• Applications
  • Rapid assessment of native/recombinant sEH activity. •  Soluble Epoxide Hydrolase activity in mammalian cell culture, and tissue lysates.
• Sample Type
  Cell lysate, tissue lysate, recombinant enzyme, purified protein
• Features Benefits
  • Rapid, simple & sensitive • Convenient & HTS • Includes specific sEH inhibitor
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Soluble Epoxide Hydrolase or sEH (EC 3.3.2.10) is a cytosolic enzyme present ubiquitously in several organs including the liver, kidney, pancreatic islets, pituitary gland, lymphoid tissues, muscles, and the gastrointestinal tract. It catalyzes the hydrolysis of epoxyeicosatrienoic acids (EETs), i.e., epoxides derived from cytochrome P-450 mediated metabolism of arachidonic acid, and forming vicinal diols. sEH plays a major role in metabolism of endogenous lipids that are implicated in pain and inflammation. BioVision’s Soluble Epoxide Hydrolase Activity Assay Kit is a microplate based fluorometric kit for measuring sEH activity in cells and tissues as well as purified protein. It is based on the ability of sEH to hydrolyze a non-fluorescent substrate to a fluorescent product. The kit includes a specific inhibitor for soluble epoxide hydrolase, since the substrate can be hydrolyzed by non-specific hydrolases that are present in cell and tissue lysates. Specific sEH activity can be obtained by subtracting the activity in presence of sEH inhibitor form the total activitys.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Soluble   Epoxide   Hydrolase   Activity   Kit   Fluorometric  
• Short name
  Soluble Epoxide Hydrolase Activity Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Soluble Epoxide Hydrolase Activity test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Product images

Similar products