Soluble Epoxide Hydrolase Inhibitor Screening Kit (Fluorometric)

• Catalog number
  K480-100
• Price
  Please ask
• Size
  100 assays

• Description
  Simplest High-Throughput Assay To Screen Thioredoxin 1 Inhibitors
• Summary
  • Detection method-Fluorescence (Ex/Em: 362/460 nm) • Applications- Screening/studying/characterizing Soluble Epoxide Hydrolase inhibitors
• Detection Method
  Fluorescence (Ex/Em: 362/460 nm)
• Species Reactivity
  Human
• Applications
  Screening/studying/characterizing soluble Epoxide Hydrolase inhibitors
• Features Benefits
  • Rapid, simple & sensitive • Convenient & HTS • Includes NCND (inhibitor control)
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Soluble Epoxide Hydrolase (EC 3.3.2.10) is a cytosolic enzyme with highest expression in the liver but present ubiquitously in several organs including the kidney, pancreatic islets, pituitary gland, lymphoid tissues, muscles, and the gastrointestinal tract. It catalyzes the hydrolysis of epoxyeicosatrienoic acids (EETs), i.e., epoxides derived from cytochrome P-450 mediated metabolism of arachidonic acid, to form vicinal diols. The EETs cause vasodilation and have anti-inflammatory actions. Since the EETs are hydrolyzed to diols through soluble epoxide hydrolase, the inhibition of this enzyme is likely to enhance the beneficial properties of EETs. This makes soluble epoxide hydrolase a very useful target for cardiovascular and pain medicine. BioVision’s Soluble Epoxide Hydrolase Inhibitor Screening Kit is a micro-plate based fluorometric kit for screening sEH inhibitors. Its assay principle is based on the hydrolysis of a non-fluorescent substrate to a fluorescent product by sEH. It uses N-Cyclohexyl-Nʹ-dodecylurea (NCND), a well-known sEH inhibitor, as positive control. The kit provides a simple and fast method to screen/ characterize potential sEH inhibitors.
• Data Sheet Link
• Additional description
  Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.

• Gene target
  Soluble   Epoxide   Hydrolase   Inhibitor   Screening   Kit   Fluorometric  
• Short name
  Soluble Epoxide Hydrolase Inhibitor Screening Kit (Fluorometric)
• Alternative name
  Soluble Epoxide Hydrolase suppressor detection reagent (fluorescence)
• Alternative technique
  kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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