Sphingomyelinase Activity Fluorometric Assay Kit

• Catalog number
  K574-100
• Price
  Please ask
• Size
  100 assays

• Description
  Simple assay to measure sphingomyelinase activity
• Summary
  • Detection method- Fluorescence (Ex/Em 535/587 nm) • Applications- Measurement of SMase activity in various tissues/cells extracts
• Detection Method
  Fluorescence (Ex/Em 535/587 nm)
• Applications
  Measurement of SMase activity in various tissues/cells extracts
• Sample Type
  • Animal tissues: brain, heart, kidney, etc. • Cell culture: Adherent or suspension cells
• Features Benefits
  • Simple, rapid & convenient • The assay can detect sphingomyleinase activity as low as 10 µU/ml in a variety of samples.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphocholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger that induces a variety of cell regulatory phenomena such as apoptosis, cell differentiation, cell proliferation and sterol homeostasis. BioVision’s SMase Fluorometric Assay Kit provides a simple and sensitive method for measuring neutral-SMase enzymatic activity using fluorescence (Ex/Em = 535/587 nm). Neutral sphingomyelinase (N-SMase) is a mg2+ sensitive enzyme that can be activated by a host of physiologically relevant and structurally diverse molecules. In this assay, SMase converts sphingomyelin substrate to phosphocholine and ceramide, which is then further utilized to produce fluorescence. This high-throughput adaptable assay kit can detect sphingomyelinase activity as low as 10 µU/ml in a variety of samples.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Sphingomyelinase   Activity   Fluorometric   Kit  
• Short name
  Sphingomyelinase Activity Fluorometric Assay Kit
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Sphingomyelinase Activity fluorescence test reagent
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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