Protease Activity Fluorometric Assay Kit

• Catalog number
  K781-100
• Price
  Please ask
• Size
  100 assays

• Description
  Highly Sensitive Assay
• Summary
  • Detection method- Fluorescence (Ex/Em 485/530 nm) • Applications- This kit can detect < 500 pg/well Trypsin present in the sample.
• Sample Type
  Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, growth medium, food samples, etc.
• Features Benefits
  • Simple procedure; takes ~60 minutes • Fast and convenient • Sensitive assay for measuring protease activity in a number of sample types
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Proteases are naturally present in all organisms. These enzymes are involved in a multitude of physiological reactions from simple digestion of food proteins to highly regulated cascades. Proteases can either break specific peptide bonds (limited proteolysis), depending on the amino acid sequence of a protein, or break down a complete peptide to amino acids (unlimited proteolysis). The activity can be a destructive change (abolishing a protein's function), an activation of a function (preform to mature form) or it can be a signal in a signaling pathway. BioVision’s Protease Activity Assay Kit is designed for the quantitative determination of proteases present in the protein sample. The assay uses fluorescein isothiocyanate (FITC)-labeled casein as a general protease substrate. The fluorescein label on the FITC-Casein is highly quenched. Upon digestion by proteases present in the sample the FITC-Casein substrate is cleaved into smaller peptides which abolishes the quenching of the fluorescence label. The fluorescence of the FITC-labeled peptide fragments is measured at Ex/Em = 485/530 nm. The kit is supplied with our Mass Spectrometry Grade (MSG), chemically stabilized Trypsin for use as a general protease control. However, other protease standard controls can also be used. This kit is easy to use and can detect < 500 pg/well Trypsin present in the sample.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Protease   Activity   Fluorometric   Kit  
• Short name
  Protease Activity Fluorometric Assay Kit
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Protease Activity fluorescence test reagent
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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