Proteinase K, Recombinant, Standard Grade

• Catalog number
  9250-100
• Price
  Please ask
• Size
  100 mg

• Synonyms
  Protease K, Endopeptidase K, Standard Grade Proteinase K, Proteinase K, Tritirachium alkaline proteinase
• Alternative_names
  Protease K, Endopeptidase K, Standard Grade Proteinase K, Proteinase K, Tritirachium alkaline proteinase
• Description
  Proteinase K, Standard Grade
• Recombinant
  True
• Source
  Recombinant Pichia pastoris
• Purity by SDS PAGE
  >90%
• Unit Definition
  One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 umole (181ug) of tyrosine per minute at pH 7.5 at 37℃.
• Molecular Weight
  28.9
• Storage Temp
  -20ºC
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized powder
• Physical form description
  White Lyophilized powder
• Reconstitution Instructions
  Soluble in water ((1–2 mg/ml)
• Background Information
  A serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It retains its activity in presence of SDS and urea. It is inactivated by diisopropyl fluorophosphates (DFP) and phenyl methane sulfonyl fluoride (PMSF). It is not inhibited by iodoacetic acid, the trypsin-specific inhibitor TLCK, the chymotrypsin-specific inhibitor TPCK, and p-chloromercuribenzoate.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  Molecular biology applications to digest unwanted proteins, such as nucleases from DNA or RNA preparations from microorganisms, cultured cells, and plants.
• Datasheet
• Gene
  Proteinase K s a broad-spectrum serine protease. The enzyme was discovered in 1974 in extracts of the fungus Engyodontium album (formerly Tritirachium album).Proteinase K is able to digest hair (keratin), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity. This enzyme belongs to Peptidase family S8. The molecular weight of Proteinase K is 28,900 Daltons (28.9 kDa).
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Proteinase   Grade  
• Short name
  Proteinase K, Recombinant, Standard Grade
• Technique
  Recombinant, Standard, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture. standards
• Alternative name
  Proteinase K, Rec., reference Grade
• Alternative technique
  rec, standards

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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