- Catalog number9250-10G
- Product nameProteinase K, Recombinant, Standard Grade
- Size10 g
- PriceAsk For Price
- SynonymsProtease K, Endopeptidase K, Standard Grade Proteinase K, Proteinase K, Tritirachium alkaline proteinase
- Alternative namesProtease K, Endopeptidase K, Standard Grade Proteinase K, Proteinase K, Tritirachium alkaline proteinase
- DescriptionProteinase K, Standard Grade
- RecombinantTrue
- SourceRecombinant Pichia pastoris
- Purity by SDS PAGE>90%
- Unit DefinitionOne unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 umole (181ug) of tyrosine per minute at pH 7.5 at 37℃.
- Molecular Weight28.9
- Storage Temp-20ºC
- ShippingGel Pack
- Shelf Life12 months
- AppearanceLyophilized powder
- Physical form descriptionWhite Lyophilized powder
- Reconstitution InstructionsSoluble in water ((1–2 mg/ml)
- Background InformationA serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It retains its activity in presence of SDS and urea. It is inactivated by diisopropyl fluorophosphates (DFP) and phenyl methane sulfonyl fluoride (PMSF). It is not inhibited by iodoacetic acid, the trypsin-specific inhibitor TLCK, the chymotrypsin-specific inhibitor TPCK, and p-chloromercuribenzoate.
- HandlingCentrifuge the vial prior to opening.
- UsageMolecular biology applications to digest unwanted proteins, such as nucleases from DNA or RNA preparations from microorganisms, cultured cells, and plants.
- Datasheet
- Gene targetProteinase K Grade
- Short nameProteinase K, Recombinant, Standard Grade
- technique filter
- Recombinant
- Standard
- TechniqueRecombinant, Standard, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture. standards
- Alternative techniquerec, standards