Proteinase K, Recombinant, Molecular Grade

• Catalog number
  9210-10G
• Price
  Please ask
• Size
  10 g

• Synonyms
  Endopeptidase K, Tritirachium alkaline proteinase
• Alternative_names
  Protease K, Endopeptidase K, Tritirachium alkaline proteinase, Molecular Biology grade Proteinase K
• Description
  A serine protease which inactivates nucleases
• Recombinant
  Yes
• Source
  Tritirachium album
• Purity by SDS PAGE
  ≥90%
• Assay
  SDS-PAGE
• Activity Specifications test method
  >30 units/mg
• Unit Definition
  One unit is defined as the amount of enzyme that will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 µmol tyrosine per min at  37°C, pH 7.5 (color by Folin-Ciocalteu reagent).
• Molecular Weight
  28.9 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized
• Physical form description
  Lyophilized with no additives
• Reconstitution Instructions
  In 50 mM Tris-HCl, 2 mM Calcium acetate, pH 8.0.
• Background Information
  A serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It retains its activity in presence of SDS and urea. It is inactivated by diisopropyl fluorophosphates (DFP) and phenyl methane sulfonyl fluoride (PMSF).
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Gene
  Proteinase K s a broad-spectrum serine protease. The enzyme was discovered in 1974 in extracts of the fungus Engyodontium album (formerly Tritirachium album).Proteinase K is able to digest hair (keratin), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity. This enzyme belongs to Peptidase family S8. The molecular weight of Proteinase K is 28,900 Daltons (28.9 kDa).
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Proteinase   Molecular   Grade  
• Short name
  Proteinase K, Recombinant, Molecular Grade
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  Proteinase K, Rec., Molecular Grade
• Alternative technique
  rec

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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