Proteinase K, Recombinant, Standard Grade
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Catalog number
9251-100
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Price
Please ask
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Size
100 ml
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Synonyms
Proteinase K, Endopeptidase K, Tritirachium alkaline proteinase, Protease K, Standard Grade Proteinase K
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Alternative_names
Proteinase K, Endopeptidase K, Tritirachium alkaline proteinase, Protease K, Standard Grade Proteinase K
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Description
Proteinase K, Standard Grade
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Recombinant
True
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Source
Recombinant Pichia pastoris
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Purity by SDS PAGE
>90%
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Unit Definition
One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 umole (181ug) of tyrosine per minute at pH 7.5 at 37℃.
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Molecular Weight
28.9
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Storage Temp
-20ºC
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Liquid
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Physical form description
Liquid
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Reconstitution Instructions
Soluble in water ((1–2 mg/ml)
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Background Information
A serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It retains its activity in presence of SDS and urea. It is inactivated by diisopropyl fluorophosphates (DFP) and phenyl methane sulfonyl fluoride (PMSF). It is not inhibited by iodoacetic acid, the trypsin-specific inhibitor TLCK, the chymotrypsin-specific inhibitor TPCK, and p-chloromercuribenzoate.
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Handling
Centrifuge the vial prior to opening.
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Usage
Molecular biology applications to digest unwanted proteins, such as nucleases from DNA or RNA preparations from microorganisms, cultured cells, and plants.
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Gene
Proteinase K s a broad-spectrum serine protease. The enzyme was discovered in 1974 in extracts of the fungus Engyodontium album (formerly Tritirachium album).Proteinase K is able to digest hair (keratin), hence, the name "Proteinase K". The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity. This enzyme belongs to Peptidase family S8. The molecular weight of Proteinase K is 28,900 Daltons (28.9 kDa).
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Short name
Proteinase K, Recombinant, Standard Grade
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Technique
Recombinant, Standard, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture. standards
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Alternative name
Proteinase K, Rec., reference Grade
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Alternative technique
rec, standards
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MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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