Choline Kinase (ChoK) Activity Assay Kit (Fluorometric)
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Catalog numberK476-100
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Price:
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Size100 assays
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DescriptionSensitive Assay, HTS
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Summary• Detection method- Fluorescence (Ex/Em 535/587 nm) • Species reactivity- Eukaryotes • Applications- The kit detects 0.5 µM choline kinase.
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Detection MethodFluorescence (Ex/Em= 535/587 nm)
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Species ReactivityEukaryotes
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ApplicationsMeasurement of choline kinase activity in different cell lines and tissues
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Sample Type• Purified Protein • Cell/tissue lysates: kidney, liver
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Features Benefits• Simple procedure; takes ~ less than 40 minutes • Fast and convenient
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Storage Conditions-20°C
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Shipping Conditionsgel pack
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Shelf life12 months
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BackgroundPyruvate kinase (PK, EC 2.7.1.40) is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Lack of pyruvate kinase will slow down the process of glycolysis which causes the disease known as pyruvate kinase deficiency. BioVision provides a simple, direct and automation-ready procedure for measuring pyruvate kinase activity in various biological samples such as blood, tissues, and culture cells, etc. In the assay, PEP and ADP were catalyzed by PK to generate pyruvate and ATP. The generated pyruvate is oxidized by pyruvate oxidase to produce color (at λ = 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the increase in color or fluorescence intensity is proportional to the increase in pyruvate amount, the PK activity can be accurately measured. The kit detects 0.1 mU/ml pyruvate kinase.
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TestBiovision supplies other types of Assays as 1.
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Gene target
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Short nameCholine Kinase (ChoK) Activity Assay Kit (Fluorometric)
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TechniqueAssay, assays
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HostAssay
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Alternative nameCholine phosphorylation catalyst (ChoK) Activity test reagent (fluorescence)
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Alternative techniquearrays, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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