Choline Oxidase Activity Assay Kit (Fluorometric)

• Catalog number
  K2052-100
• Price
  Please ask
• Size
  100 assays

• Description
  To determine the activity of choline oxidase in samples
• Summary
  BioVison’s Choline Oxidase Activity Assay Kit is the first, commercially available kit to determine the activity of choline oxidase in samples. In this assay, choline oxidase oxidizes choline to generate hydrogen peroxide, which then reacts with the probe to generate a strong fluorescent signal measured at Ex/Em = 535/587 nm. The fluorescent signal is directly proportional to the choline oxidase activity in the samples. The assay is simple, fast, reproducible and is high throughput adaptable. The kit can detect as low as 0.2 mU of choline oxidase activity under assay conditions.
• Detection Method
  Fluorescence (Ex/Em = 535/587 nm)
• Species Reactivity
  Bacteria
• Applications
  Measurement of choline oxidase activity
• Sample Type
  Purified Protein,Bacterial cell lysates
• Features Benefits
  The assay is simple, fast, reproducible and is high throughput adaptable.,The first, commercially available kit to determine the activity of choline oxidase in samples.,The kit can detect as low as 0.2 mU of choline oxidase activity under assay conditions.
• Storage Conditions
  -20°C
• Shipping Conditions
  Gel Pack
• Shelf life
  12 months
• Background
  Choline Oxidase (E.C. 1.1.3.17) belongs to the oxidoreductase family and is present in plants, bacteria, fungi and mammals. It is a catabolic enzyme that catalyzes the chemical reaction of choline and oxygen into betaine glycine and hydrogen peroxide with betaine aldehyde as an intermediate. The enzyme participates in glycine, serine, and threonine metabolism and is commonly used as a tool for the enzymatic determination of acetylcholinesterase, phospholipase D, phosphatidylcholine-specific phospholipase C, sphingomyelinase etc. Betaine glycine is a well known osmoprotectant and can help plants and bacteria to survive dry, cold and salt stresses. As a result, choline oxidase has been engineered genetically to produce transgenic plants such as tomato, rice and tobacco with increased stress tolerance and productivity. Additionally, accumulation of betaine glycine has been observed in bacteria such as Staphylocccus aureus, Pseudomonas aeruginosa and Listeria monocytogenes thereby suggesting that drugs capable of inhibiting choline oxidase activity will have potential anti-bacterial activity. BioVison’s Choline Oxidase Activity Assay Kit is the first, commercially available kit to determine the activity of choline oxidase in samples. In this assay, choline oxidase oxidizes choline to generate hydrogen peroxide, which then reacts with the probe to generate a strong fluorescent signal measured at Ex/Em = 535/587 nm. The fluorescent signal is directly proportional to the choline oxidase activity in the samples. The assay is simple, fast, reproducible and is high throughput adaptable. The kit can detect as low as 0.2 mU of choline oxidase activity under assay conditions.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.

• Gene target
  Choline   Oxidase   Activity   Kit   Fluorometric  
• Short name
  Choline Oxidase Activity Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  Choline Oxidase Activity test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Product images

Similar products